Abstract
Clostridium histolyticum grew on glycine, arginine, or threonine as sole substrate. Arginine degradation preceded that of glycine and partially inhibited that of threonine when two amino acids were present. Each amino acid seemed to be individually catabolized, not by a Stickland type of reaction. Glycine fermentation required the presence of complex ingredients. Therefore, an effect of selenite on glycine catabolism could only be demonstrated after scavenging selenium contamination by preculturing Peptostreptococcus glycinophilus in that medium. C. acidiurici was not suited as selenium accumulating organism as C. histolyticum was inhibited by the residual uric acid. Arginine catabolism was unaffected by seleniuum depriviation. The labelling pattern obtained in acetate after incubation of C. histolyticum with [1-14C]- or [2-14C]glycine strongly indicated the metabolism of glycine via the glycine reductase pathway.
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References
Barker HA (1949) Le métabolisme des gazes par les bactéries anaerobiés. Ann Inst Past Paris 77:361–379
Barker HA (1981) Amino acid degradation by anaerobic bacteria. Ann Rev Biochem 50:23–40
Beerens H (1953/54) Amélioation des techniques d'étude et d'identification des bactéries anaerobiés. Ann Inst Past Lille 6:36–52
Beerens H, Guillaume J, Ponte C (1956) Production d'ammoniaque et toxicité de Clostridium histolyticum cultivé en milieu gélantiné. Ann Inst Past Paris 91:407–410
Bulmash J, Weaver RH (1957) Nutritional studies with Clostridium histolyticum. J Bacteriol 74:110
Cardon BP, Barker HA (1947) Amino acid fermentations by Clostridium propionicum and Diplococcus glycinophilus. Arch Biochem 12:165–180
Ceriotti G, Spandrio L (1957) An improved method for microdetermination of arginine by use of 8-hydroxyquinoline. Biochem J 66:603–607
Cunin R, Glansdorff N, Pierard A, Stalon V (1986) Biosynthesis and metabolism of arginine in bacteria. Microbiol Rev 50:314–352
Dürre P, Andreesen JR (1982) Selenium-dependent growth and glycine fermentation by Clostridium purinolyticum. J Gen Microbiol 128:1457–1466
Dürre P, Andreesen JR (1983) Purine and glycine metabolism by purinolytic clostridia. J Bacteriol 154:192–199
Dürre P, Spahr R, Andreesen JR (1983) Glycine fermentation via glycine reductase in Peptococcus glycinophilus and Peptococcus magnus. Arch Microbiol 134:127–135
Elsden SR, Hilton MG (1978) Volatile acid production from threonine, valine, leucine and isoleucine by clostridia. Arch Microbiol 117:165–172
Elsden SR, Hilton MG (1979) Amino acid utilization patterns in clostridial taxonomy. Arch Microbiol 123:137–141
Freudenberg W (1987) Charakterisierung des Glycin-Decarboxylase/Synthase Enzymkomplexes aus Eubacterium acidaminophilum. PhD thesis, Univ Göttingen
Golovchenko, NP, Belokopytov BF, Akimenko VK (1982) Threonine catabolism in the bacterium Clostridium sticklandii. Biochemistry (USSR) 47:969–974
Guillaume J, Beerens H, Osteux H (1956) Production de gaz carbonique et fermentation du glycocolle par Clostridium histolyticum. Ann Inst Past Paris 91:721–726
Lebertz H (1984) Selenabhängiger Glycin-Stoffwechsel bei anaeroben Bakterien und vergleichende Untersuchungen zur Glycin-Reduktase und zur Glycin Decarboxylase. PhD thesis, Univ Göttingen
Mead GC (1971) The amino acid-fermenting clostridia. J Gen Microbiol 67:47–56
Rieth M (1987) Untersuchungen zur selenabhängigen Glycinreduktase aus Eubacterium acidaminophilum. PhD thesis, Univ Göttingen
Sardesai VM, Provido HS (1970) The determination of glycine in biological fluids. Clin Chim Acta 29:67–71
Schiefer-Ullrich H (1984) Harnsäureabbau im Hühnerdarm durch anaerobe Bakterien. PhD thesis, Univ Göttingen
Schiefer-Ullrich H, Wagner R, Dürre P, Andreesen JR (1985) Comparative studies on physiology and axonomy of obligately purinolytic clostridia. Arch Microbiol 138:345–353
Smith LDS (1975) The pathogenic anaerobic bacteria. 2nd ed. CC Thomas Publisher, Springfield, IL
Stadtman TC (1954) On the metabolism of an amino acid fermenting clostridium. J Bacteriol 67:314–320
Stadtman TC (1980) Selenium enzymes. Ann Rev Biochem 49:93–110
Tziaka C (1987) Untersuchungen zur Charakterisierung von Stämmen der Gattungen Peptococcus und Peptostreptococcus. PhD thesis, Univ Göttingen
Van Wart HE, Steinbrink DR (1985) Complementary substrate specificities of class I and class II collagenase from Clostridium histolyticum. Biochemistry 24:6520–6526
Waber JL, Wood HG (1979) Mechanism of acetate synthesis from CO2 by Clostridium acidiurici. J Bacteriol 140:468–478
Warren GH, Gray J (1961) A simplified culture medium for the production of collagenase. Nature 192:755–756
Zindel U, Freudenberg W, Rieth M, Andreesen JR, Schnell J, Widdel F (1988) Eubacterium acidaminophilum sp. nov., a versatile anaerobe in amino acid utilization depending on the withdrawal or presence of hydrogen gas or formate. Description and enzymatic studies. Arch Microbiol (in press)
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Lebertz, H., Andreesen, J.R. Glycine fermentation by Clostridium histolyticum . Arch. Microbiol. 150, 11–14 (1988). https://doi.org/10.1007/BF00409710
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DOI: https://doi.org/10.1007/BF00409710