Abstract
Cultures of Chromatium vinosum, devoid of sulfur globules, were supplemented with sulfide and incubated under anoxic conditions in the light. The concentrations of sulfide, polysulfides, thiosulfate, polythionates and elemental sulfur (sulfur rings) were monitored for 3 days by ion-chromatography and reversed-phase HPLC. While sulfide disappeared rapidly, thiosulfate and elemental sulfur (S6, S7 S8 rings) were formed. After sulfide depletion, the concentration of thiosulfate decreased fairly rapidly, but elemental sulfur was oxidized very slowly to sulfate. Neither polysulfides (S 2−x ), polythionates (SnO 2−6 , n=4–6), nor other polysulfur compounds could be detected, which is in accordance with the fact that sulfide-grown cells were able to oxidize polysulfide without lag. The nature of the intracellular sulfur globules is discussed.
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References
Feher F, Laue W (1956) Beiträge zur Chemie des Schwefels. XXIX. Über die Darstellung von Rohsulfanen. Z Anorg Allg Chem 288:103–112
Fischer U (1984) Cytochromes and iron sulfur proteins in sulfur metabolism of phototrophic sulfur bacteria. Sulfur, its significance for chemistry, for the geo-, bio-, and the cosmosphere and technology. In: Müller A, Krebs B (eds) Studies in inorganic chemistry, vol 5. Elsevier, Amsterdam, pp 383–407
Gerischer H (1949) Über die Auflösungsgeschwindigkeit von Schwefel in Sulfid- und Polysulfidlösungen. Z Anorg Chem 259:220–224
Guerrero R, Mas J, Pedrós-Alió C (1984) Buoyant density changes due to intracellular content of sulfur in Chromatium warmingii and Chromatium vinosum. Arch Microbiol 137:350–356
Guerrero R, Pedrós-Alió C, Schmidt TM, Mas J (1985) A survey of buoyant density of microorganisms in pure cultures and natural samples. Microbiologia (Spain) 1:53–65
Hansen CJ (1933) Die Einwirkung von Schwefelwasserstoff und Sulfiden auf Polythionate. Ber Dtsch Chem Ges 66:817–825
Hageage GJ Jr, Eanes ED, Gherna RL (1970) X-ray diffraction studies of the sulfur globules accumulated by Chromatium species. J Bacteriol 101:464–469
Herbert D, Phipps PJ, Strange RE (1971) Chemical analysis of microbial cells In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press, New York, pp 209–344
Kurtenacker A, Goldbach E (1927) Über die Analyse von Polythionatlösungen. Z Anorg Allg Chem 166:177–189
Kurtenacker A, Kaufmann M (1925) Über die Einwirkung von Schwefelwasserstoff auf die Polythionate. Z Anorg Allg Chem 148:256–264
Licht S, Hodes G, Manassen J (1986) Numerical analysis of aqueous polysulfide solutions and its application to cadmium chalcogenide/polysulfide photoelectrochemical solar cells Inorg Chem 25:2486–2489
Lowry OH, Rosenbrough NJ, Farr AL, Radall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193:265–275
Mas J, Pedrós-Alió C, Guerrerro R (1985) Mathematical model for determining the effects of intracytoplasmatic inclusions on volume and density of microorganisms. J Bacteriol 164:749–756
Mas J, Van Gemerden H (1987) Influence of sulfur accumulation and composition of sulfur globule on cell volume and buoyant density of Chromatium vinosum. Arch Microbiol 146:362–369
Nicolson GL, Schmidt GL (1971) Structure of the Chromatium sulfur particle and its protein membrane. J Bacteriol 105:1142–1148
Rosental S (1930) Die Dielektrizitätskonstante unterkühlten Schwefels und einiger Schwefellösungen. Z Phys 66:652–656
Schedel M (1978) Untersuchungen zur anaeroben Oxidation reduzierter Schwefelverbindungen durch Thiobacillus denitrificans, Chromatium vinosum und Chlorobium limicola. Ph D Thesis, University of Bonn, FRG
Schmidt GL, Nicolson GL, Kamen MD (1971) Composition of the sulfur particle of Chromatium strain D. J Bacteriol 105:1137–1141
Schwarzenbach G, Fischer A (1960) Die Acidität der Sulfane und die Zusammensetzung wässriger Polysulfidlösungen. Helv Chim Acta 43:1365–1390
Smith AJ (1966) The role of tetrathionate in the oxidation of thiosulphate by Chromatium sp. strain D. J Gen Microbiol 42:371–380
Steudel R (1984) Neue Entwicklungen in der Chemie des Schwefels und des Selens. Nova Acta Leopoldina NF 264:59–231
Steudel R (1989) On the nature of the “elemental sulfur” (So) produced by sulfur-oxidizing bacteria — a model for So globules. In: Schlegel HG, Bowien B (eds) Biology of autotrophic bacteria. Science Tech Publ, Madison, and Springer, Berlin Heidelberg New York, pp 289–303
Steudel R, Holdt G (1986) Ion-pair chromatographic separation of polythionates SnO 2−6 with up to thirteen sulphur atoms. J Chromatogr 361:379–384
Steudel R, Göbel T, Holdt G (1988) The molecular composition of hydrophilic sulfur sols prepared by acid decomposition of thiosulfate. Z Naturforsch B 43:203–218
Steudel R, Holdt G, Göbel T (1989) Ion-pair chromatographic separation of inorganic sulfur anions including polysulfide. J Chromatogr 475:442–446
Steudel R, Holdt G, Göbel T, Hazeu W (1987) Chromatographic separation of higher polythionates SnO 2−6 (n=3...22) and their detection in cultures of Thiobacillus ferrooxidans: Molecular composition of bacterial sulfur secretions. Angew Chem Int Ed Engl 26:151–153
Strauss R, Steudel R (1987) Schnelle chromatographische Trennung und Bestimmung der Schwefel-Homocyclen Sn (n=6–28) mittels HPLC. Fresenius'Z Anal Chem 326:543–546
Tebbe FN, Wasserman E, Peet WG, Vatvars A, Hayman AC (1982) Composition of elemental sulfur in solution: Equilibrium of S6, S7 and S8 at ambient temperatures. J Am Chem Soc 104:4971–4872
Then J (1984) Beiträge zur Sulfidoxidation durch Ectothiorhodospira abdelmalekii und Ectothiorhodospira halochloris. Ph D Thesis, University of Bonn, FRG
Trüper HG (1984) Phototrophic bacteria and their sulfur metabolism. Sulfur, its significance for chemistry, for the geo-, bio-, and the cosmosphere and technology. In: Müller A, Krebs B (eds) Studies in inorganic chemistry, vol 5. Elsevier, Amsterdam, pp 367–382
Trüper HG (1989) Physiology and biochemistry of phototrophic bacteria. In: Schlegel HG, Bowien B (eds) Biology of autotrophic bacteria. Science Tech Publ, Madison and Springer, Berlin Heidelberg New York, pp 267–281
Trüper HG, Fischer U (1982) Anaerobic oxidation of sulphur compounds as electron donors for bacterial photosynthesis. Phil Trans R Soc Lond B 298:529–542
Trüper HG, Schlegel HG (1964) Sulphur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii. J Microbiol Serol 30:225–238
Van Gemerden H (1968) Growth measurements in Chromatium cultures. Arch Mikrobiol 64:103–110
Van Gemerden H (1984) Coexistence of organisms competing for the same substrate: an example among the purple sulfur bacteria. Micr Ecol 1:104–119
Van Gemerden H (1984) The sulfide affinity of phototrophic bacteria in relation to the location of elemental sulfur. Arch Microbiol 139:289–294
Van Gemerden H (1987) Competition between purple sulfur bacteria and green sulfur bacteria: role of sulfide, sulfur and polysulfides. Acta Acad Aboensis 47:13–27
Van Gemerden H, Beeftink HH (1978) Specific rates of substrate oxidation and product formation in autotrophically growing Chromatium vinosum cultures. Arch Microbiol 119:135–143
Van Gemerden H, Tughan CS, De Wit R, Herbert RA (1989) Laminated microbial ecosystems on sheltered beaches in Scapa Flow, Orkney Islands. FEMS Microbiol Ecol 62: 87–102
Van Niel CB (1936) On the metabolism of Thiorhodaceae. Arch Mikrobiol 7: 323–358
Visscher PT, Van Gemerden H (1988) Growth of Chlorobium limicola f. thiofulfatophilum on polysulfides. In: Olson JM, Ormerod JG, Amesz J, Stackebrandt E, Trüper HG (eds) Green photosynthetic bacteria. Plenum Press, New York, pp 287–294
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Steudel, R., Holdt, G., Visscher, P.T. et al. Search for polythionates in cultures of Chromatium vinosum after sulfide incubation. Arch. Microbiol. 153, 432–437 (1990). https://doi.org/10.1007/BF00248423
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DOI: https://doi.org/10.1007/BF00248423