Abstract
A bacterial strain producing cephalosporin acylases was isolated from soil. The morphological and physiological properties of this strain suggest that it belongs to the genus Arthrobacter, and the isolate was therefore designated Arthrobacter strain 45-8A. Substrate specificity of the enzyme was examined. The enzyme can convert both cephalosporin C and 7β-(4-carboxylbutan-amino)cephalosporanic acid to 7-aminocephalosporanic acid. An interesting feature of the acylases is their temperature-dependent regulation. Activity of acylases was detected in strain 45-8A grown at temperature below 30 °C, but was not observed at higher temperature. Arthrobacter strain 45-8A did not exhibit β-lactamase activity, even though its resistance to cephalosporin C was very strong (>2000 μg/ml). This is quite beneficial for its application in the manufacture of 7-aminocephalosporanic acd.
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Abbreviations
- NBHAB:
-
2-Nitro-5-(6-bromohexanoylamino)-benzoic acid
- NIPAB:
-
2-Nitro-5-phenylacetaminobenzoic acid
- CPC:
-
cephalosporin C
- GL-7ACA:
-
7β-(4-carboxybutanamino)cephalosporanic acid
- 6-APA:
-
aminopenicillanic acid
- 7-ACA:
-
7-aminocephalosporanic acid
- PDAB:
-
p-Dimethylaminobenzaldehyde
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Zhang, Q.J., Xu, W.X. Morphological, physiological and enzymatic characteristics of cephalosporin acylase-producing Arthrobacter strain 45-8A. Arch. Microbiol. 159, 392–395 (1993). https://doi.org/10.1007/BF00290923
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DOI: https://doi.org/10.1007/BF00290923