Abstract
Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the generaPseudomonas, Moraxella, Arthrobacter andAeromonas. A hydroxylamine-cytochromec oxidoreductase activity was detected in periplasmic fractions of thePseudomonas andAeromonas spp. and in total soluble fractions of theArthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochromec oxidoreductase was purified from thePseudomonas species and shown to be similar to hydroxylaminecytochromec oxidoreductase ofParacoccus denitrificans.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- AMO :
-
Ammonia monoxygenase
- HAO :
-
Hydroxylamine-cytochromec oxidoreductase
References
Arciero D, Hooper AB (1993) Hydroxylamine oxidoreductase fromNitrosomonas is a multimer of an octa-heme subunit. J Biol Chem 268:14645–14654
Bergmann DJ, Hooper AB (1995) The primary structure of cytochrome P460 ofNitrosomonas europaea: presence of ac-heme binding motif. FEBS Lett 353:324–326
Bergstrom DW, Tenula M, Beauchamp EG (1994) Increase in nitrous oxide production in soil induced by ammonium and organic carbon. Biol Fertil Soils 18:1–6
Berks BC, Moir JWB, Ferguson SJ, Richardson DJ (1995) Enzyme and associated electron transfer systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions. Biochim Biophys Acta 1232:97–173
Carter J, Hsaio YH, Spiro S, Richardson DJ (1995) Soil and sediment bacteria capable of aerobic nitrate respiration. Appl Environ Microbiol 61:2852–2858
Castignetti D, Hollocher TC (1984) Heterotrophic nitrification among denitrifiers. Appl Environ Microbiol 47:620–623
Coleman JK, Cornish-Bowden A, Cole JA (1978) Purification and properties of nitrite reductase fromEscherichia coli K12. Biochem J 175:483–493
Hoppert M, Mahony TJ, Mayer F (1995) Quaternary structure of the hydroxylamine oxidoreductase fromNitrosomonas europaea. Arch Microbiol 163:300–306
Jetten MSM, De Bruijn P, Kuenen JG (1996) Hydroxylamine metabolism inPseudomonas PB16: involvement of a novel hydroxylamine oxidoreductase. Antonie Van Leeuwenhoek (in press)
Kurokawa M, Fukumori Y, Yamanaka T (1985) A hydroxylaminecytochromec oxidoreductase occurs in the heterotrophic nitrifierArthrobacter globiformis. Plant Cell Physiol 26:1439–1442
Moir JWB, Barratta D, Richardson DJ, Ferguson SJ (1993) The purification of acd 1-type nitrite reductase and the absence of a copper-type nitrite reductase from the aerobic denitrifierThiosphaera pantotropha; the role of pseudoazurin as electron donor. Eur J Biochem 212:377–385
Moir JWB, Wehrfritz J-M, Spiro S, Richardson DJ (1996) Characterisation of a novel non-haem iron hydroxylamine oxidase from the bacteriumParacoccus denitrificans. Biochem J 319: 823–827
Paul JW, Beauchamp EG, Zhang X (1993) Nitrous and nitric oxide emissions during nitrification and denitrification from manureamended soil in the laboratory. Can J Soil Sci 73:539–553
Robertson LA, Kuenen GJ (1988) Ecology of nitrification and denitrification. In: Cole JA, Ferguson SJ (eds) The nitrogen and sulphur cycles, 42nd Symposium of the Society for General Microbiology. Cambridge University Press, Cambridge
Robertson LA, Kuenen GJ (1990) Combined heterotrophic nitrification and aerobic denitrification inThiosphaera pantotropha and other bacteria. Antonie van Leeuwenhoek 57:139–152
Samyn B, Berks BC, Page MD, Ferguson SJ, Van Beeumen JJ (1994) Characterisation of the amino acid sequence of cytochromec-550 fromThiosphaera pantotropha. Eur J Biochem 219:585–594
Wehrfritz J-M, Richardson DJ, Spiro S (1993) Partial purification of hydroxylamine oxidase fromThiosphaera pantotropha: identification of electron acceptors that couple nitrification to denitrification. FEBS Lett 335:246–250
Wood PM (1988) Monooxygenase and free radical mechanisms for biological ammonia oxidation. In: Cole JA, Ferguson SJ (eds) The nitrogen and sulphur cycles, 42nd Symposium of the Society for General Microbiology. Cambridge University Press, Cambridge
Zahn JA, Duncan C, DiSpirito AA (1994) Oxidation of hydroxylamine by cytochrome P-460 of the obligate methylotrophMethylococcus capsulatus Bath. J Bacteriol 176:5879–5887
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wehrfritz, JM., Carter, J.P., Spiro, S. et al. Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochromec oxidoreductase from aPseudomonas species. Arch. Microbiol. 166, 421–424 (1996). https://doi.org/10.1007/BF01682991
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01682991