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Verwertung von Pyrimidinderivaten durch Hydrogenomonas facilis

I. Intermediärprodukte und Enzyme des Cytosinabbaues

Utilization of pyrimidine derivatives by Hydrogenomonas facilis

I. Intermediates and enzymes of cytosine degradation

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Summary

Dihydrouracil, 3-ureidopropionate and β-alanine, intermediates involved in the reductive degradation of cytosine, were utilized as a carbon and nitrogen source by cells of Hydrogenomonas facilis. Barbiturate and malonate, intermediates of the oxidative pathway, were not utilized. Uracil was converted to dihydrouracil, 3-ureidopropionate and β-alanine during incubation with extracts from cells grown with cytosine as a nitrogen source. Barbiturate and urea were not detected under these conditions. The enzymes cytosine deaminase, dihydrouracil dehydrogenase, dihydrouracil hydrase and 3-ureidopropionase but not uracil oxidase were demonstrated in cell-free extracts from cells grown with cytosine. The specific activity of dihydrouracil dehydrogenase and dihydrouracil hydrase but not of cytosine deaminase was significantly higher in extracts from cytosine grown cells, as compared with extracts from cells grown with ammonia. These data indicate that cytosine and uracil undergo a reductive degradation in cells of Hydrogenomonas facilis.

Zusammenfassung

Dihydrouracil, 3-Ureidopropionat und β-Alanin, die Intermediärprodukte des reduktiven Cytosinabbaues, wurden durch Zellen von Hydrogenomonas facilis als Kohlenstoff- und Stickstoffquelle verwertet, Barbitursäure und Malonsäure, die Intermediärprodukte des oxidativen Abbaues, dagegen nicht. Während der Inkubation mit Extrakten aus Zellen, die mit Cytosin als N-Quelle gewachsen waren, wurde Uracil zu Dihydrouracil, 3-Ureidopropionat und β-Alanin umgesetzt. Barbitursäure und Harnstoff waren hierbei nicht nachweisbar. Nach Anzucht mit Cytosin waren die Enzyme Cytosin-Desaminase, Dihydrouracil-Dehydrogenase, Dihydrouracil-Hydrase und 3-Ureidopropionase, nicht aber Uracil-Oxidase in zellfreien Extrakten nachweisbar. Gegenüber den mit NH4Cl gewachsenen Zellen zeigten die mit Cytosin herangezogenen Zellen eine deutlich erhöhte spezifische Aktivität an Dihydrouracil-Dehydrogenase und Dihydrouracil-Hydrase, nicht aber an Cytosin-Desaminase. Diesen Befunden zufolge unterliegen die Pyrimidinderivate Cytosin und Uracil in Hydrogenomonas facilis einem reduktive Abbau.

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Krämer, J., Kaltwasser, H. Verwertung von Pyrimidinderivaten durch Hydrogenomonas facilis . Archiv. Mikrobiol. 68, 227–235 (1969). https://doi.org/10.1007/BF00409915

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  • DOI: https://doi.org/10.1007/BF00409915

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