Skip to main content
Log in

Inhibition of nitrite assimilation by uncouplers of phosphorylation

  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

Carbonyl cyanide m-chlorophenylhydrazone (CCCP) and pentachlorophenol (PCP), two powerful uncouplers of phosphorylation, specifically inhibit the assimilation of nitrite in the course of nitrate reduction. These results support our former conclusion that high-energy phosphate is involved in the metabolism of nitrite.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ahmad, J., Morris, I.: Inhibition of nitrate and nitrite reduction by 2,4-dinitrophenol in Ankistrodesmus. Arch. Mikrobiol. 56, 219–224 (1967).

    Google Scholar 

  • —— The effects of 2,4-dinitrophenol and other uncoupling agents on the assimilation of nitrate and nitrite by Chlorella. Biochim. biophys. Acta (Amst.) 162, 32–38 (1968).

    Google Scholar 

  • Czygan, F.-C.: Zur Frage der Zwischenprodukte der Nitratreduktion bei Grünalgen. Planta (Berl.). 64, 301–311 (1965).

    Google Scholar 

  • Del Campo, F. F., Ramírez, J. M., Paneque, A., Losada, M.: Ferredoxin and the dark and light reduction of dinitrophenol. Biochem. biophys. Res. Commun. 22, 547–553 (1966).

    Google Scholar 

  • Heytler, P. G.: Uncoupling of oxidative phosphorylation by carbonyl cyanide phenylhydrazones. I. Some characteristics of m-Cl-CCP action on mitochondria and chloroplasts. Biochemistry 2, 357–361 (1963).

    Google Scholar 

  • Kessler, E.: Über die Wirkung von 2,4-Dinitrophenol auf Nitratreduktion und Atmung von Grünalgen. Planta (Berl.) 45, 94–105 (1955).

    Google Scholar 

  • —: Nitrate assimilation by plants. Ann. Rev. Plant Physiol. 15, 57–72 (1964).

    Google Scholar 

  • —, Bücker, W.: Über die Wirkung von Arsenat auf Nitratreduktion, Atmung und Photosynthese von Grünalgen. Planta (Berl.) 55, 512–524 (1960).

    Google Scholar 

  • —, Czygan, F.-C.: Physiologische und biochemische Beiträge zur Taxonomie der Gattung Chlorella. IV. Verwertung organischer Stickstoffverbindungen. Arch. Mikrobiol. 70, 211–216 (1970).

    Google Scholar 

  • Nason, A., Takahashi, H.: Inorganic nitrogen metabolism. Ann. Rev. Microbiol. 12, 203–246 (1958).

    Google Scholar 

  • Ramírez, J. M., Del Campo, F. F., Paneque, A., Losada, M.: Ferredoxin-nitrite reductase from spinach. Biochim. biophys. Acta (Amst.) 118, 58–71 (1966).

    Google Scholar 

  • Ritenour, G. L., Joy, K. W., Bunning, J., Hageman, R. H.: Intracellular localization of nitrate reductase, nitrite reductase, and glutamic acid dehydrogenase in green leaf tissue. Plant Physiol. 42, 233–237 (1967).

    Google Scholar 

  • Schrader, L. E., Beevers, L., Hageman, R. H.: Differential effects of chloramphenicol on the induction of nitrate and nitrite reductase in green leaf tissue. Biochem. biophys. Res. Commun. 26, 14–17 (1967).

    Google Scholar 

  • Weinbach, E. C.: The effect of pentachlorophenol on oxidative phosphorylation. J. biol. Chem. 210 545–550 (1954).

    Google Scholar 

  • Wessels, J. S. C.: Mechanism of the reduction of organic nitro compounds by chloroplasts. Biochim. biophys. Acta (Amst.) 109, 357–371 (1965).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kessler, E., Hofmann, A. & Zumft, W.G. Inhibition of nitrite assimilation by uncouplers of phosphorylation. Archiv. Mikrobiol. 72, 23–26 (1970). https://doi.org/10.1007/BF00411011

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00411011

Keywords

Navigation