Summary
Sclerotia of Sclerotinia sclerotiorum (Lib.) D By. were obtained from commercial pea-and bean-cleaning operations or grown on potato-dextrose agar and synthetic glucose-and sucrose-salts agar media. The crude fat (ether extract) content of sclerotia varied from 0.8 to 1.5%. Extraction and fractionation of the lipids followed by gas chromatographic analysis showed that sclerotia from pea cleanings contained one predominant hydrocarbon which was absent from sclerotia produced in the laboratory. Sclerotia from natural sources and grown in the laboratory contained a similar distribution of C18 unsaturated free fatty acids, however, quantitative differences were noted. Palmitic, oleic and linoleic were the major free fatty acids of the laboratory-grown sclerotia while a high proportion of linoleic acid was also found in sclerotia from natural sources. Sclerotia were fractionated into water-soluble and water-insoluble fractions. After acid hydrolysis of the waterinsoluble fraction, both fractions were analyzed for amino acids. Twenty-one compounds, including 2 unknowns, were detected in the soluble fraction. The hydrolyzates contained 19 amino acids, including the same 2 unknowns. Two compounds tentatively identified as ornithine and γ-aminobutyric acid were found only in the water-soluble fraction. The relative amino acid composition of the water-insoluble fraction of sclerotia from various sources was fairly constant but the arginine content decreased on the synthetic media.
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Bharuca, K. E., Gunstone, F. D.: Vegetable oils. VI. Component acids of ergot oil. J. chem. Soc. 1957, 610–614.
Chet, I., Henis, Y., Mitchell, R.: Chemical composition of hyphal and sclerotial walls of Sclerotium rolfsii Sacc. Canad. J. Microbiol. 13, 137–141 (1967).
Eglinton, G., Gonzales, A. G., Hamilton, R. J., Raphael, R. A.: Hydrocarbon constitutents of the wax coatings of plant leaves: a taxonomic survey. Phytochemistry 1, 89–102 (1962).
—, Hamilton, R. J.: The distribution of alkanes. In: Chemical plant taxonomy, pp. 187–217 (ed. by T. Swain). New York: Academic Press 1963.
Herbin, G. A., Robins, P. A.: Patterns of variation and development in leaf wax alkanes. Phytochemistry 8, 1985–1998 (1969).
Howell, D. McB., Fergus, C. L.: The component fatty acids found in sclerotia of Sclerotium rolfsii. Canad. J. Microbiol. 10, 616–618 (1964).
Laseter, J. L., Hess, W. M., Weete, J. D., Stocks, D. L., Weber, D. J.: Chemotaxonomic and ultrastructural studies on three species of Tilletia occurring on wheat. Canad. J. Microbiol. 14, 1149–1154 (1968a).
—, Weete, J. D., Weber, D. J.: Alkanes, fatty acid methyl esters, and free fatty acids in surface wax of Ustilago maydis. Phytochemistry 7, 1177–1181 (1968b).
Le Tourneau, D.: Trehalose and acyclic polyols in Sclerotinia sclerotiorum. Mycologia (N. Y.) 58, 934–942 (1966).
Morrison, W. R., Smith, L. M.: Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron trifluoride-methanol. J. Lipid Res. 5, 600–608 (1964).
Obreiter, J. B., Sproston, T.: Amino acids in relation to thermoperiod-cycling in Sclerotinia trifoliorum. Phytopath. 54, 1395–1397 (1964)
Óro, J., Laseter, J. L., Weber, D. J.: Alkanes in fungal spores. Science 154, 399–400 (1966).
—, Tornabene, T. G., Nooner, D. W., Gelpi, E.: Aliphatic hydrocarbons and fatty acids of some marine and freshwater microorganisms. J. Bact. 93, 1811–1818 (1967).
Purdy, L. H.: A broader concept of the species Sclerotinia sclerotiorum based on variability. Phytopath. 45, 421–427 (1955).
Spackmann, D. H., Stein, W. H., Moore, S.: Automatic recording apparatus for use in the chromatography of amino acids. Analyt. Chem. 30, 1190–1206 (1958).
Tornabene, T. G., Gelpi, E., Óro, J.: Identification of fatty acids and aliphatic hydrocarbons in Sarcina lutea by gas chromatography and combined gas chromatography-mass spectrometry. J. Bact. 94, 333–343 (1967).
Weber, D. J.: Role of proline and glutamic acid metabolism in spore germination of Rhizopus arrhizus. Phytopath. 56, 118–123 (1966).
Weete, J. D., Laseter, J. L., Weber, D. J., Hess, W. M., Stocks, D. L.: Hydrocarbons, fatty acids, and ultrastructure of smut spores. Phytopath. 59, 545–548 (1969).
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Weete, J.D., Weber, D.J. & Le Tourneau, D. Hydrocarbons, free fatty acids, and amino acids of sclerotia of Sclerotinia sclerotiorum . Archiv. Mikrobiol. 75, 59–66 (1970). https://doi.org/10.1007/BF00412093
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DOI: https://doi.org/10.1007/BF00412093