Summary
The level of riboflavin synthetase in growing cultures of Bacillus subtilis is controlled by repression. The enzyme level is derepressed in flavinogenic mutants of the microorganism. Riboflavin-deficient mutants accumulating 6,7-dimethyl-8-ribityllumazine are devoid of riboflavin synthetase.
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Adelberg, E. A., Mandel, M., Chen, G. C. C.: Optimal conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine in Escherichia coli K 12. Biochem. biophys. Res. Commun. 18, 788–795 (1965).
Bacher, A., Lingens, F.: Biosynthesis of riboflavin. Formation of 2,5-diamino-6-hydroxy-4-(1′-D-ribitylamino) pyrimidine in a riboflavin auxotroph. J. biol. Chem. 245, 4647–4652 (1970).
Bacher, A., Lingens, F.: Biosynthesis of riboflavin. Formation of 6-hydroxy-2,4,5-triaminopyrimidine in rib. mutants of Saccharomyces cerevisiae. J. biol. Chem. 246, 7018–7022 (1971).
Bresler, S. E., Kalinin, V. L., Krivisky, A. S., Perumov, D. A.: A mutant of Bacillus subtilis synthesizing considerable quantities of riboflavin. Genetika 5 (2), 133–138 (1969).
Bresler, S. E., Cherepenko, E. I., Chernik, T. P., Kalinin, V. L., Perumov, D. A.: Study on the riboflavin operon in Bacillus subtilis. I. Genetic mapping of the linkage group. Genetika 6 (5), 116–124 (1970).
Bresler, S. E., Glazunov, E. A., Perumov, D. A.: Study on the riboflavin operon in Bacillus subtilis. IV. Regulation of biosynthesis of riboflavin synthetase. Study of riboflavin transport through the cell membrane. Genetika 8 (2), 109–118 (1972).
Konishi, S., Kageyama, K., Shiro, T.: Accumulation of 6,7-dimethyl-8-ribityllumazine by auxotrophic mutant of Bacillus subtilis. Agr. Biol. Chem. 33, 90–95 (1969).
Oltmanns, O., Bacher, A.: Biosynthesis of riboflavine in Saccharomyces cerevisiae: the role of genes rib1 and rib7. J. Bact. 110, 818–822 (1972).
Plaut, G. W. E., Beach, R. L., Aogaichi, T.: Studies on the mechanism of elimination of protons from the methyl groups of 6,7-dimethyl-8-ribityllumazine by riboflavin synthetase. Biochemistry 9, 771–785 (1970).
Spizizen, J.: Transformation of biochemically deficient strains of Bacillus subtilis by desoxyribonucleate. Proc. nat. Acad. Sci. (Wash.) 44, 1072–1078 (1958).
Wilson, A. C., Pardee, A. B.: Regulation of flavin synthesis by Escherichia coli. J. gen. Microbiol. 28, 283–303 (1962).
Winestock, C. H., Plaut, G. W. E.: Synthesis and properties of certain substituted lumazines. J. org. Chem. 26, 4456–4462 (1961).
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Bacher, A., Eggers, U. & Lingens, F. Genetic control of riboflavin synthetase in Bacillus subtilis . Archiv. Mikrobiol. 89, 73–77 (1973). https://doi.org/10.1007/BF00409401
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DOI: https://doi.org/10.1007/BF00409401