Summary
Individual cells of Flexibacter aurantiacus CW7 and Hyphomicrobium vulgare ZV580 orientate themselves perpendicularly to the interface in air-water, oil-water and solid-water systems. Electrostatic phenomena probably are not involved in this orientation, since no evidence was found of any localized distribution of positively-charged ionogenic groups on the bacterial surface. It is suggested that the orientation results from a relatively hydrophobic portion of each cell being rejected from the aqueous phase of the system. This property also may be related to the formation of rosettes by these bacteria. Electron micrographs of thin sections of cells sorbed to araldite blocks show that the cell proper is not in contact with the solid surface, but is anchored to it by extracellular adhesive material. The extracellular materials may be of a polysaccharide nature.
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References
Baier, R. E., Shafrin, E. G., Zisman, W. A.: Adhesion: mechanisms that assist or impede it. Science 172, 1360–1368 (1968).
Brock, T. D.: The habitat of Leucothrix mucor, a widespread marine microorganism. Limnol. Oceanogr. 11, 303–307 (1966).
Christie, A. O., Evans, L. V., Shaw, M.: Studies on the ship-fouling alga Enteromorpha. II. The effect of certain enzymes on the adhesion of zoospores. Ann. Bot. N.S. 34, 467–482 (1970).
Conti, S. F., Hirsch, P.: Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bact. 89, 503–512 (1965).
Corpe, W. A.: Attachment of marine bacteria to solid surfaces. In: R. S. Manly, Ed., Adhesion in biological systems, pp. 73–87. New York: Academic Press 1970a.
Corpe, W. A.: An acid polysaccharide produced by a primary film-forming marine bacterium. Devel. in Indust. Microbiol. 11, 402–412 (1970b).
Deinema, M. H., Zevenhuisen, L. P. T. M.: Formation of cellulose fibrils by Gram-negative bacteria and their role in bacterial flocculation. Arch. Mikrobiol. 78, 42–57 (1971).
Gibbons, R. J., van Houte, J.: Selective bacterial adhesion to oral epithelial surfaces and its role as an ecological determinant. Infect. Immun. 3, 567–573 (1971).
Harold, R., Stanier, R. Y.: The genera Leucothrix and Thiothrix. Bact. Rev. 19, 49–54 (1955).
Hirsch, P., Conti, S. F.: Biology of budding bacteria. II. Growth and nutrition of Hyphomicrobium spp. Arch. Mikrobiol. 48, 358–367 (1964).
Hirsch, P., Pankratz, S. H.: Study of bacterial populations in natural environments by use of submerged electron microscope grids. Z. allg. Mikrobiol. 10, 589–605 (1970).
Lewin, R. A.: A classification of flexibacteria. J. gen. Microbiol. 58, 189–206 (1969).
Lewin, R. A., Lounsbery, D. M.: Isolation, cultivation and characterization of flexibacteria. J. gen. Mikrobiol. 58, 145–170 (1969).
Marshall, K. C.: Electrophoretic properties of fast- and slow-growing species of Rhizobium. Aust. J. biol. Sci. 20, 429–438 (1967).
Marshall, K. C.: Sorptive interactions between soil particles and microorganisms. In: A. D. McLaren, J. J. Skujins, Eds., Soil biochemistry, vol. II, pp. 409–445. New York: Marcel Dekker 1971.
Marshall, K. C., Stout, R., Mitchell, R.: Mechanisms of the initial events in the sorption of marine bacteria to surfaces. J. gen. Microbiol. 68, 337–348 (1971a).
Marshall, K. C., Stout, R., Mitchell, R.: Selective sorption of bacteria from seawater. Canad. J. Microbiol. 17, 1413–1416 (1971b).
Meadows, P. S.: The attachment of bacteria to solid surfaces. Arch. Mikrobiol. 75, 374–381 (1971).
Mudd, S., Mudd, E. B. H.: The penetration of bacteria through capillary spaces. IV. A kinetic mechanism in interfaces. J. exp. Med. 40, 633–645 (1924a).
Mudd, S., Mudd, E. B. H.: Certain interfacial tension relations and the behaviour of bacteria in films. J. exp. Med. 40, 647–660 (1924b).
Poindexter, J. S.: Biological properties and classification of the Caulobacter group. Bact. Rev. 28, 231–295 (1964).
Stefanson, R. C.: Effect of periodate and pyrophosphate on the seasonal changes in aggregate stabilization. Aust. J. Soil Sci. 9, 33–41 (1971).
Taylor, A. C.: Adhesion of cells to surfaces. In: R. S. Manly, Ed., Adhesion in biological systems, pp. 51–71. New York: Academic Press 1970.
Tyler, P. A., Marshall, K. C.: Form and function in manganese-oxidizing bacteria. Arch. Mikrobiol. 56, 344–353 (1967).
van Oss, C. J., Gillman, C. F.: Phagocytosis as a surface phenomenon. I. Contact angles and phagocytosis of non-opsonized bacteria. J. Reticuloendothelial Soc. (in press).
Veres, I.: Negative electrical charge of the surface of bull sperm. Mikroskopie 23, 166–169 (1968).
Zavarzin, G. A.: The life cycle and nuclear apparatus in Hyphomicrobium vulgare Stutz. and Hartleb. Mikrobiologiya (English transl.) 29, 38–42 (1960).
Zvyagintsev, D. G.: Adsorption of microorganisms by glass surfaces. Mikrobiologiya (English transl.) 28, 104–108 (1959).
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Marshall, K.C., Cruickshank, R.H. Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Archiv. Mikrobiol. 91, 29–40 (1973). https://doi.org/10.1007/BF00409536
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DOI: https://doi.org/10.1007/BF00409536