Abstract
The kinetics of xenobiotic biotransformation was measured in single, isolated, perifused liver cells with microspectrofluorometry using highly sensitive photodetection systems. A program of further applications to biochemical toxicological problems is presented.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bücher, T., Sies, H.: Mitochondrial and cytosolic redox states in perfused rat liver: Methods and problems in metabolic compartmentation. In: Use of isolated liver cells and kidney tubules in metabolic studies. Tager, J. M., Söling, H. D., Williamson, J. R., (eds.). Amsterdam: North-Holland Publ. Comp. 1976
Burke, M. D., Hallman, H.: Microfluorimetric analysis of the cytochrome P 448 associated, ethoxyresorufin O-deethylase activities of individual isolated rat hepatocytes. Biochem. Pharmacol. 27, 1539–1544 (1978)
Burke, M. D., Mayer, R. T.: Ethoxyresorufin: Direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab. Dispos. 2, 583–588 (1974)
Burke, M. D., Orrenius, S.: The effect of albumin on the metabolism of ethoxyresorufin through O-deethylation and sulfate-conjugation using isolated rat hepatocytes. Biochem. Pharmacol. 27, 1533–1538 (1978)
Cherry, R. J.: Measurement of protein rotational diffusion in membranes by flash photolysis. In: Methods in enzymology, Vol. 54, part E. Fleischer, S., Parker, L. (eds.), pp. 47–61. New York: Academic Press 1978
Kohen, E., Thorell, B., Kohen, C., Salmon, J. M.: Studies on metabolic events in localized compartments of the living cell by rapid microspectrofluorometry. Adv. Biol. Med. Phys. 15, 271–297 (1977)
Kunz, W., Appel, K. E., Rickart, R., Schwarz, M., Stöckle, G.: Enhancement and inhibition of carcinogenic effectiveness of nitrosamines. In: Primary liver tumors. Remmer, H., Bolt, H. M., Bannasch, P., Popper, H. (eds.), pp. 261–283. Lancaster: MTP Press 1978
Sasse, D., Katz, N., Jungermann, K.: Functional heterogeneity of rat liver parenchyma and of isolated hepatocytes. FEBS Lett. 57, 83–88 (1975)
Schlessinger, J., Koppel, D. E., Axelrod, D., Jacobsen, K., Webb, W. W., Elson, E. L.: Lateral transport on cell membranes: Mobility of concanavalin A receptors on myoblasts. Proc. Natl. Acad. Sci. USA 73, 2409–2413 (1976)
Seglen, P. O.: Preparation of isolated rat liver cells. In: Methods in cell biology, Vol. 13. Prescott, D. M. (ed.), pp. 29–83. New York: Academic Press 1976
Thurman, R. G., Marazzo, D. P., Jones, L. S., Kauffman, F. C.: The continuous kinetic determination of p-nitroanisole O-demethylation in hemoglobin-free perfused rat liver. J. Pharmacol. Exp. Ther. 201, 498–506 (197■)
Ullrich, V., Weber, P.: The O-dealkylation of 7-ethoxycoumarin by liver microsomes. Hoppe-Seylers Z. physiol. Chem. 353, 1171–1177 (1972)
Vaz, W. L. C., Austin, R. H., Vogel, H.: The rotational diffusion of cytochrome b5 in lipid bilayer membranes. Influence of the lipid physical state. Biophys. J. 26, 415–426 (1979)
Yguerabide, J.: Nanosecond fluorescence spectroscopy of macromolecules. In: Methods in enzymology, Vol. 26. Fleischer, S., Parker, L. (eds.), pp. 498–578. New York: Academic Press 1972
Author information
Authors and Affiliations
Additional information
Part of Dissertation of these authors
Rights and permissions
About this article
Cite this article
Stier, A., Nolte, K.H., Schlenker, A. et al. Toxicological studies of liver cells by microspectrofluorometry. Arch. Toxicol. 44, 45–54 (1980). https://doi.org/10.1007/BF00303182
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00303182