Abstract
Thymidine transport and phosphorylation were investigated in isolated rat hepatocytes and AS 30 D hepatoma cells. In contrast to hepatoma cells, hepatocytes exhibited a minimum of thymidine phosphorylation due to a 100-fold smaller thymidine kinase activity. In hepatocytes thymidine is transported by two transport systems: a specific concentrative “high affinity” system and an unspecific non-concentrative “low affinity” system. In hepatoma cells only the “low affinity” system could be detected. A single dose of 20 or 50 mg diethylnitrosamine/kg body weight induced in hepatocytes a remarkable increase of thymidine kinase activity and a decrease of the transport by the “high affinity” system. Thymidine transport and phosphorylation by hepatocytes are considered to be sensitive markers for early recognition of toxin-induced liver regeneration.
Zusammenfassung
Thymidintransport und -phosphorylierung wurden in isolierten Rattenhepatozyten und AS 30 D-Hepatomzellen untersucht. Hepatozyten wiesen im Gegensatz zu Hepatomzellen aufgrund einer 100fach niedrigeren Thymidinkinaseaktivität eine äußerst niedrige Thymidinphosphorylierungsrate auf. In Hepatozyten wurde Thymidin durch zwei Transportsysteme aufgenommen: ein spezifisches, konzentratives „high affinity“ System und ein unspezifisches, nichtkonzentratives „low affinity“ System. In ATP-freien Hepatomzellen konnte nur das „low affinity“ System nachgewiesen werden. Eine einmalige Dosis von 20 bzw. 50 mg Diäthylnitrosamin/kg Körpergewicht bewirkte in Hepatozyten einen Anstieg der Thymidinkinaseaktivität und eine Verminderung des Thymidintransports über das „high affinity“ System. Thymidintransport und -phosphorylierung in Hepatozyten erwiesen sich als sensitives System zur frühen Erkennung beginnender Leberregeneration nach toxischer Vorschädigung.
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Ungemach, F.R., Hegner, D. Thymidine transport in hepatocytes. Arch. Toxicol. 44, 167–173 (1980). https://doi.org/10.1007/BF00303193
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DOI: https://doi.org/10.1007/BF00303193