Abstract
The well known fact that the activity of δ-aminolevulinic acid dehydratase (ALAD: EC 4.2.1.24) is reduced in red cells of animals with lead poisoning was found to be upset, by using a modified method of Gibson's original procedure, for determination of activated ALAD activity.
The modified method involves addition of 0.2 mM Zn2+ and then preheating the enzyme solution at 60° C for 5 min before following Gibson's original procedure. With this methodological modification, the ALAD activity of erythrocytes of rats poisoned with lead was found increased. Furthermore, the enzyme was purified from the peripheral blood of lead-poisoned rats. ALAD protein in peripheral blood was also determined by single radial immuno diffusion using rabbit anti-serum raised against rat liver ALAD. As the result, the ALAD activity obtained from the modified method was found to be directly proportional to the absolute amount of enzyme proteins determined both by chemically and immunochemically.
The modified method for measuring true ALAD content in blood cells in lead poisoning is more reliable than previous ones.
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Kajimoto, M., Kondo, M., Niwa, M. et al. Increase of δ-Aminolevulinic Acid Dehydratase (ALAD) in rat erythrocytes in lead poisoning. Arch Toxicol 52, 1–11 (1983). https://doi.org/10.1007/BF00317977
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DOI: https://doi.org/10.1007/BF00317977