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The influence of blood flow on the absorption of l- and d-phenylalanine from the jejunum of the rat

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Summary

  1. 1.

    Jejunal loops of anaesthetized rats were perfused (single pass and recirculation) with buffered solutions containing l-phenylalanine (about 30 μM and 30 mM) and d-phenylalanine (about 30 μM). The blood flow, the disappearance rate (from the intestinal lumen) and the appearance rate (in the intestinal venous blood) were determined simultaneously, the accumulation in the gut wall at the end of the experiments.

  2. 2.

    At a constant intermediate blood flow the absorption of l- and d-phenylalanine decreased with time. A decrease of blood flow from about 1.6 to 0.25 ml min−1g−1 wet tissue weight diminished the appearance and the disappearance rate of both isomers. An increase of blood flow from 0.25 to 1.6 ml min−1g−1 caused no increase of absorption; on the contrary the appearance and disappearance rate decreased a little. The different dependence of absorption when decreasing and increasing the blood flow rate may be caused by a changed pattern of blood flow in the layers of the gut wall, i.e. the flow rate in the villi may be reduced while the flow rate in the entire wall remains unchanged.

  3. 3.

    In the single pass perfusion experiments the appearance rate of l-phenylalanine increased proportionally to the luminal concentration (30 μM and 30 mM), while the accumulation was relatively smaller at the high concentration. In the recirculation experiments the appearance rate and the accumulation of l-phenylalanine did not increase proportionally.

  4. 4.

    The increase in perfusion rate, from 0.1 (single pass perfusion) to 2–3 and 10 ml min−1 (recirculation), caused an increase of l-phenylalanine absorption and tissue accumulation.

  5. 5.

    At a luminal concentration of about 30 μM the appearance rates of l- and d-phenylalanine were equal, while the tissue accumulation was smaller for the d-isomer.

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  1. Pharmakologisches Institut der Universität Tübingen, Germany

    D. Winne

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Winne, D. The influence of blood flow on the absorption of l- and d-phenylalanine from the jejunum of the rat. Naunyn-Schmiedeberg's Arch. Pharmacol. 277, 113–138 (1973). https://doi.org/10.1007/BF00501154

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