Zusammenfassung
Aus überlebenden Froschdärmen durch Diffusion gewonnener Darmstoff wurde säulen- und dünnschichtchromatographisch mit Prostaglandin verglichen.
Die Untersuchungen zeigen, daß die Hauptwirkung dieses Darmstoffpräparates auf dem Gehalt an den Hydroxyfettsäuren Prostaglandin E1 (PGE1) und F1 (PGF1) beruht. Daneben enthält es einen geringen Anteil eines weiteren, unidentifizierten Lipoids.
Da saure Phosphatide in dem aus Dialysaten gewonnenen Darmstoff nicht nachweisbar waren, wohl aber in Darmstoffpräparaten, die durch Kochen oder Lösungsmittelextraktion erhalten wurden, wird angenommen, daß die sauren Phosphatide im lebenden Gewebe strukturgebunden sind. Sie können daher nur lokale Funktionen ausüben. Polare Fettsäuren wie die Prostaglandine dagegen können in diffusionsfähigem Zustand auftreten und potentiell humorale Wirkstoffe sein.
Summary
Darmstoff obtained from the bath fluid of the isolated gastro-intestinal tract of frogs was compared with prostaglandin by column and thin layer chromatography. The smooth muscle effect of this Darmstoff preparation is mainly due to its content of the prostaglandins PGE1 and PGF1. In addition another lipid component constributes weakly to the activity.
Acidic phosphatides which are major biologically active constituents of lipid extracts or hot water extracts of horse intestine, are not present — or only to a small degree — in dialysates of surviving frog intestine. This suggests that in living tissue the smooth muscle contracting phosphatides are bound to cellular structures, and extractable only after disintegration of the tissue. Acidic phosphatides can, therefore, only have locally restricted functions. Polar fatty acids like prostaglandins, on the other hand, occur in a diffusible state and can be humoral transmitters, potentially.
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Herrn Professor Dr. F. Timm zum 70. Geburtstag gewidmet.
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Gast-Professor am Institut. Derzeitige Adresse: Fukushima Medical College, Department of Pharmacology, Fukushima, Japan.
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Suzuki, T., Vogt, W. Prostaglandine in einem Darmstoffpräparat aus Froschdarm. Naunyn - Schmiedebergs Arch 252, 68–78 (1965). https://doi.org/10.1007/BF00246431
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DOI: https://doi.org/10.1007/BF00246431