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The effects of isoprenaline and tyramine on the 45calcium uptake, the total calcium content and the contraction force of isolated guinea-pig atria in dependence on different extracellular hydrogen ion concentrations

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Summary

Isolated, electrically stimulated left guinea-pig atria were incubated at 30° C in a Tyrode solution, the hydrogen ion concentration of which was varied by altering the percentage of the aerating CO2/O2-mixture. The increase of the extracellular hydrogen ion concentration from pH 8.0 to pH 7.0 caused a reduction of the 45calcium uptake and of the contraction force. The positive inotropic effect and the concomitantly occuring increased 45calcium uptake induced by isoprenaline and tyramine were markedly diminished at the lower pH, the response to tyramine being more affected by increased hydrogen ion concentration than that of isoprenaline. This is possibly due to a decrease in noradrenaline available at pH 7.0 for release by tyramine. Isoprenaline and tyramine enhanced the relative specific 45calcium activity (RSA) without altering the total tissue calcium content. On the contrary, the increase of the extracellular calcium concentration caused an augmentation of the relative specific 45calcium activity and the total tissue calcium content.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Düsseldorf, Düsseldorf, Germany

    M. Morgenstern, E. Noack & E. Köhler

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  1. M. Morgenstern
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  2. E. Noack
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  3. E. Köhler
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Supported by the Deutsche Forschungsgemeinschaft.

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Morgenstern, M., Noack, E. & Köhler, E. The effects of isoprenaline and tyramine on the 45calcium uptake, the total calcium content and the contraction force of isolated guinea-pig atria in dependence on different extracellular hydrogen ion concentrations. Naunyn-Schmiedeberg's Arch. Pharmacol. 274, 125–137 (1972). https://doi.org/10.1007/BF00501847

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  • DOI: https://doi.org/10.1007/BF00501847

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