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Suppression of 3H-acetylcholine release from primary nerve cell cultures by tetanus and botulinum-A toxin

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Summary

Primary nerve cell cultures derived from embryonic rat central nervous system form [3H]ACh from exogenous [3H]Ch, and release it upon potassium depolarization.

Pretreatment of the cultures with botulinum-A toxin or tetanus toxin diminishes the cellular accumulation of [3H]ACh. Poisoning the cultures during the period of [3H]Ch uptake fails to lower [3H]ACh formation.

Dependent on dosage, both toxins suppress the release of [3H]ACh upon potassium depolarization.

Heat-denaturated toxins as well as tetanus toxin preincubated with tetanus antitoxin were without effect.

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Abbreviations

BTA:

Botulinum-A toxin

TET:

Tetanus toxin

ACh:

Acetylcholine

Ch:

Choline

[3H]Ch:

[3H-Methyl]-choline

[3H]ACh:

Acetyl [3H-Methyl]-choline

DMEM:

Dulbecco's modified Eagle's medium

ChAc (EC 2.3.1.6.):

Choline acetyl transferase

[14C]ACh:

[1-14C]Acetylcholine

PBS:

Phosphate buffered saline

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Authors and Affiliations

  1. Pharmakologisches Institut der Justus Liebig-Universität Giessen, Frankfurter Strasse 107, D-6300, Lahn-Giessen 1, Germany

    H. Bigalke, W. Dimpfel & E. Habermann

Authors
  1. H. Bigalke
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  2. W. Dimpfel
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  3. E. Habermann
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This work was supported by DFG via Sonderforschungsbereich 47

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Bigalke, H., Dimpfel, W. & Habermann, E. Suppression of 3H-acetylcholine release from primary nerve cell cultures by tetanus and botulinum-A toxin. Naunyn-Schmiedeberg's Arch. Pharmacol. 303, 133–138 (1978). https://doi.org/10.1007/BF00508058

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  • DOI: https://doi.org/10.1007/BF00508058

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