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Influence of microelectrophoretically applied acetylcholine on the responsiveness of hippocampal and lateral geniculate neurones

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Zusammenfassung

  1. 1.

    Neurone im Gebiet des Hippocampus und des Corpus geniculatum laterale der mit Chloralose-Urethan narkotisierten Katze wurden mit mehrkanüligen Mikroelektroden auf ihre Ansprechbarkeit auf mikroelektrophoretisch appliziertes Acetylcholin und auf optische Reizung untersucht.

  2. 2.

    Im ventralen Hippocampus und im Corpus geniculatum laterale wurden 60% aller untersuchten Neurone durch Acetylcholin aktiviert.

  3. 3.

    Die Neurone des Hippocampus wurden nach relative kurzer Latenz, oft weniger als 1 sec, aktiviert. Neurone des Corpus geniculatum laterale dagegen zeigten eine Latenz von 2–12 sec. Die Zeit nach der Mikroelektrophorese bis zur Wiedererreichung der normalen Aktivität betrug 10–20 sec für beide Gebiete.

  4. 4.

    Die Acetylcholin-Mikroelektrophorese verstärkte die Reizantwort der Neurone des Hippocampus und des Corpus geniculatum laterale auf visuelle Reizung. Die nach Phenobarbital abgeschwächte Antwort auf visuelle Reizung wurde durch Acetylcholin-Mikroelektrophorese wieder verstärkt.

Summary

  1. 1.

    Hippocampal and lateral geniculate neurones in anaesthetized cats (Chloralose-Urethane) were examined with multibarrelled microelectrodes, testing their responsiveness to microelectrophoretic application of acetylcholine and photic stimulation.

  2. 2.

    In the ventral hippocampus and in the lateral geniculate body 60% of all neurones responded to acetylcholine.

  3. 3.

    Hippocampal neurones were activated with relatively short delay, often less than 1 sec. By contrast, lateral geniculate neurones responded with a latency period of 2–12 sec. The recovery time after acetylcholine-microelectrophoresis was found to be 10–20 sec in both regions.

  4. 4.

    The response of hippocampal and lateral geniculate neurones to photic stimulation was enhanced by local application of acetylcholine; even during phenobarbital depression the photic responses were restored by acetylcholinemicroelectrophoresis.

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Authors and Affiliations

  1. Department of Experimental Medicine, F. Hoffmann-La Roche & Co. Ltd., Basle

    Felix A. Steiner

  2. Institute for Brain Research, University of Zurich, Zurich

    Felix A. Steiner

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  1. Felix A. Steiner
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Steiner, F.A. Influence of microelectrophoretically applied acetylcholine on the responsiveness of hippocampal and lateral geniculate neurones. Pflugers Arch. 303, 173–180 (1968). https://doi.org/10.1007/BF00592634

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  • DOI: https://doi.org/10.1007/BF00592634

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