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Wash out kinetics of Na from the transport pool to the epithelial and corium side of the frog skin

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Summary

The wash out kinetics of Na-24 out of the transport pool of the frog skin were followed simultaneously to the epithelial and corium side in control experiments and after inhibition of the Na transport with ouabain. A perfusion system was used which allowed changes of the perfusion chambers within 5 sec between labelling and tracer wash out.

In the control, the Na of the transport pool is washed out with a half time of 2.9±0.3 min and 3.2±0.7 min to the corium and the epithelial side, respectively.

After ouabain, the half times increased to 8.9±0.9 min and 8.6±1.1 min for the corium and the epithelial wash out. From this observation it is concluded that the transport Na is distributed in an intracellular compartment.

The ratio of the two tracer effluxes from the transport pool to the corium and the epithelial sidej *23 /j *21 is 30±8 in the control and 4.9±2.0 after ouabain.

The wash out to the epithelial side shows an additional fast component (half times 0.70±0.08 min in the control and 0.68±0.13 after ouabain) which is interpreted as wash out of an extracellular compartment. This view is supported by measurements of the wash out of Inulin C-14.

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References

  1. Aceves, J., Erlij, D.: Sodium transport across the isolated epithelium of the frog skin. J. Physiol. (Lond.)212, 195 (1971).

    Google Scholar 

  2. Cereijido, M., Curran, P. F.: Intracellular electrical potentials in the frog skin. J. gen. Physiol.48, 543 (1965).

    Google Scholar 

  3. Cereijido, M., Herrera, F. C., Flanigan, W. J., Curran, P. F.: The influence of Na concentration on Na-transport across frog skin. J. gen. Physiol.47, 879 (1964).

    Google Scholar 

  4. Cereijido, M., Rotunno, C. A.: Transport and distribution of sodium across frog skin. J. Physiol. (Lond.)190, 481 (1967).

    Google Scholar 

  5. Curran, P. F., Herrera, F. C., Flanigan, W. J.: The effect of Ca and antidiuretic hormone on Na transport. J. gen. Physiol.46, 1011 (1963).

    Google Scholar 

  6. Dörge, A., Nagel, W.: Effect of Amiloride on sodium transport in frog skin. II. Sodium transport pool and unidirectional fluxes. Pflügers Arch.321, 91 (1970).

    Google Scholar 

  7. Ehrlich, E. N., Crabbe, J.: The mechanism of action of Amipramizide. Pflügers Arch. ges. Physiol.301, 79 (1968).

    Google Scholar 

  8. Gehring, K., Dörge, A., Nagel, W., Thurau, K.: The use of the scanning electron microscope in connection with a solid state detector for analysis and localization of electrolytes in biological tissue. (In press.)

  9. Herrera, F. C.: Action of ouabain on sodium transport in the toad urinary bladder. Amer. J. Physiol.210, 980 (1966).

    Google Scholar 

  10. Hoshiko, T., Ussing, H. H.: The kinetics of Na24 flux across amphibian skin and bladder. Acta physiol. scand.49, 74 (1960).

    Google Scholar 

  11. Imamura, A., Takeda, H., Jasaki, N.: The accumulation of sodium and calcium in a specific layer of frog skin. J. cell. comp. Physiol.66, 221 (1965).

    Google Scholar 

  12. Nagel, W., Dörge, A.: A study of different sodium compartments and the transepithelial sodium fluxes of the frog skin with the use of ouabain. Pflügers Arch.324, 267 (1971).

    Google Scholar 

  13. Parsons, P. H., Hoshiko, T.: Separation of epithelium from frog skin and rapid washout of22Na. J. gen. Physiol.57, 254 (1971).

    Google Scholar 

  14. Schoffeniels, E.: An isolated single electroplax preparation. II. Improved preparation for studying ion flux. Biochim. biophys. Acta (Amst.)26, 585 (1957).

    Google Scholar 

  15. Ussing, H. H., Windhager, E. E.: Nature of shunt path and active sodium transport path through frog skin epithelium. Acta physiol. scand.61, 484 (1964).

    Google Scholar 

  16. Vanatta, J. C., Bryant, L. A.: Compartmentation of the sodium transport pool of the toad bladder. Proc. Soc. exp. Biol. (N.Y.)133, 385 (1970).

    Google Scholar 

  17. Zerahn, K.: Nature and localization of the sodium pool during active transport in the isolated frog skin. Acta physiol. scand.77, 272 (1969).

    Google Scholar 

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Authors and Affiliations

  1. Physiologisches Institut der Universität München, Pettenkoferstr. 12, D-8000, München, Germany

    Adolf Dörge & Wolfram Nagel

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  1. Adolf Dörge
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  2. Wolfram Nagel
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Supported by the Deutsche Forschungsgemeinschaft.

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Dörge, A., Nagel, W. Wash out kinetics of Na from the transport pool to the epithelial and corium side of the frog skin. Pflugers Arch. 337, 285–297 (1972). https://doi.org/10.1007/BF00586646

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  • DOI: https://doi.org/10.1007/BF00586646

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