Summary
Reynafarje's (1963) spectrophotometric method was modified for the study of myoglobin concentration in rat hearts. The influence of age and body weight was evaluated and the age was found to be better correlated with myoglobin concentration than the weight. The influence of simulated altitude of 3500 m was studied in animals exposed to hypoxia at the age of about 34 days and in animals born in the low pressure chamber. In the first group a higher myoglobin concentration was found but only in the right ventricle, together with unilateral hypertrophy, as compared with control animals. In the group of animals born at simulated altitude a severe cardiomegaly developed which was most pronounced in the right ventricle. The myoglobin concentration in the hearts from this group was unchanged in the right ventricle and was lower in the left ventricle and septum.
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Anthony, A., Ackerman, E., Strother, G. K.: Effects of altitude acclimatization on rat myoglobin. Changes in myoglobin content of skeletal and cardiac muscle. Amer. J. Physiol.196, 512–516 (1959).
Bowen, W. J., Eads, H. J.: Effects of 18,000 feet simulated altitude on the myoglobin content of dogs. Amer. J. Physiol.159, 77–82 (1949).
Clark, R. T., Jr., Criuscuolo, D., Coulson, C. K.: Effects of 20,000 ft. simulated altitude on myoglobin content of animals with and without exercise. Fed. Proc.11, 25 (1952).
Costa, L. E., Taquini, A. C.: Effect of chronic hypoxia on myoglobin, cytochromes and ubiquinone levels in the rat. Acta physiol. lat.-amer.20, 103–109 (1970).
Criuscuolo, D., Clark, R. T., Jr., Mefferd, R. B., Jr.: Effects of low and high iron supplementation on hypoxic rats. Amer. J. Physiol.180, 215–218 (1955).
Fulton, R. M., Hutchinson, E. C., Jones, A. M.: Ventricular weight in cardiac hypertrophy. Brit. Heart J.14, 413–420 (1952).
Hurtado, A., Rotta, A., Merino, C., Pons, J.: Studies of myohemoglobin at high altitudes. Amer. J. med. Sci.194, 708–713 (1937).
Kreuzer, F.: Facilitated diffusion of oxygen and its possible significance; a review. Respir. Physiol.9, 1–30 (1970).
Poel, W. E.: Effect of anoxic anoxia on myoglobin concentration in striated muscle. Amer. J. Physiol.156, 44–51 (1949).
Poupa, O., Krofta, K., Prochazka, J., Turek, Z.: Acclimation to simulated high altitude and acute cardiac necrosis. Fed. Proc.25, 1243–1246 (1966).
Rakušan, K., Rádl, J., Poupa, O.: The distribution and content of myoglobin in the heart of the rat during postnatal development. Physiol. Bohemoslov.14, 317–319 (1965).
Reynafarje, B.: Simplified method for the determination of myoglobin. J. Lab. clin. Med.61, 138–145 (1963).
Snedecor, G. W., Cochran, W. G.: Statistical methods. Ames, Iowa: Iowa State University Press 1967.
Tappan, D. V., Reynafarje, B.: Tissue pigment manifestation of adaptation to high altitudes. Amer. J. Physiol.190, 99–103 (1957).
Timiras, P. S.: Comparison of growth and development of the rat at high altitude and at sea level. In: The physiological effects of high altitude, ed. W. H. Weihe, pp. 21–30. Oxford: Pergamon Press 1964.
Turek, Z., Frans, A., Kreuzer, F.: Hypoxic pulmonary steady-state diffusing capacity for CO and alveolar-arterial O2 pressure differences in growing rats after adaptation to a simulated altitude of 3500 m. Pflügers Arch.335, 1–9 (1972a).
Turek, Z., Ringnalda, B. E. M., Hoofd, L. J. C., Frans, A., Kreuzer, F.: Cardiac output, arterial and mixed-venous O2 saturation, and blood O2 dissociation curve in growing rats adapted to a simulated altitude of 3500 m. Pflügers Arch.335, 10–18 (1972b).
Turek, Z., Grandtner, M., Kreuzer, F.: Cardiac hypertrophy, capillary and muscle fiber density, muscle fiber diameter, capillary radius and diffusion distance in the myocardium of growing rats adapted to a simulated altitude of 3500 m. Pflügers Arch.335, 19–28 (1972c).
Vaughan, B. E., Pace, N.: Changes in myoglobin content of the high altitude acclimatized rat. Amer. J. Physiol.155, 549–556 (1956).
Wittenberg, J. B.: Myoglobin-facilitated oxygen diffusion: Role of myoglobin in oxygen entry into muscle. Physiol. Rev.50, 559–636 (1970).
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Turek, Z., Ringnalda, B.E.M., Grandtner, M. et al. Myoglobin distribution in the heart of growing rats exposed to a simulated altitude of 3500 m in their youth or born in the low pressure chamber. Pflugers Arch. 340, 1–10 (1973). https://doi.org/10.1007/BF00592192
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DOI: https://doi.org/10.1007/BF00592192