Summary
Renal cortical and plasma renin activities and the electron microscopic appearance of juxtaglomerular epitheloid cells were investigated in the unclamped renin-depleted kidney of rats with two-kidney-Goldblatt hypertension after removal of the clamped kidney. Within 24 h the renal renin content fell to still lower values, on the 2d day, it rose to one half its normal value, on the 7th day it reached its control value. Renin activity of plasma did not significantly differ from zero until the 3d day after removing the clamped kidney, reached 20% of normal on the 7th day and normal values between the 7th and the 21 st day. Sparse secretory granules appeared in the remaining renal cortex 12 h after removal of the clamped kidney. After 24 h secretory granules were formed by confluence of Golgi vesicles. After 2 days cisternae appeared in the rough-surfaced endoplasmic reticulum. After 4 days the electron-microscopic picture became normal. Renin appears to be elaborated in the rough-surfaced endoplasmic reticulum and concentrated into granules by the Golgi-apparatus. A fraction of the renal renin does not appear to be available for secretion.
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Barajas, L.: The development and ultrastructure of the juxtaglomerular cell granule. J. Ultrastruct. Res.15, 400–413 (1966)
Biava, C., West, M.: Lipofuscin—like granules in vascular smooth muscle and juxtaglomerular cells of human kidneys. Amer. J. Path.47, 287–313 (1965)
Bucher, O., Reale, E.: Zur elektronenmikroskopischen Untersuchung der juxtaglomerulären Spezialeinrichtungen der Niere. I.: Mitteilung, Problemstellung und erste Beobachtungen. Z. Zellforsch.54, 167–181 (1961)
Bucher, O., Riedel, B.: Der juxtaglomeruläre Apparat der Niere. Hippokrates36, 857–865 (1965)
Bucher, O., Riedel, B.: L'appareil juxtaglomérulaire du rein. C. R. Ass. Anat. 50 e Réunion à Lausanne, 1965. Bull. Ass. Anat.130, 43–77 (1966)
Cook, W. F.: Cellular localisation of renin. In: Kidney Hormones, J. W. Fisher, Ed., pp. 117–128. London-New York: Academic Press 1971
Gross, F.: Renin stores in the kidney and plasma renin activity. In: Kidney Hormones, J. W. Fisher, Ed., pp. 93–116. London-New York: Academic Press 1971
Gross, F., Brunner, H., Ziegler, M.: Renin-angiotensin system, aldosterone and sodium balance. Recent Progr. Hormone Res.21, 119–177 (1965)
Haefeli, L., Peters, G.: Rôle du système rénine-angiotensine dans le déclenchement de la soif. J. Urol. Néphrol.76, 1011–1013 (1970)
Hartroft, P. M.: The juxtaglomerular complex as an endocrine gland. In: Endocrine Pathology, J. M. B. Bloodworth, Ed., pp. 641–677. Baltimore: Williams and Wilkins 1968.
Helmchen, U., Kneissler, U., Churchill, P., Peters-Haefeli, L., Schaechtelin, G. Peters, G.: Plasma renin activity in renal hypertensive rats. Pflügers Arch.332, 232–238 (1972)
Heptinstall, R. H.: The role of the juxtaglomerular apparatus in experimental renal hypertension in the rat. Lab. Invest.14, 2150–2159 (1966)
Hinglais, N., Sultan, C., Ducrot, H.: Recherches sur l'ultrastructure de l'appareil juxtaglomérulaire au cours de sténoses de l'artère rénale chez l'homme et chez le rat. II. Morphogénèse des granulations. Actualités néphrol. Hôp. Necker1966, 97–107
Jamieson, J. D., Palade, G. E.: Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex. J. Cell Biol.34, 577–596 (1967a)
Peters-Haefeli, L.: Renal cortical renin activity and renin secretion at rest and in response to hemorrhage. Amer. J. Physiol.221, 1331–1338 (1971)
Peters, G., Schaechtelin G.: Renin, angiotensin and the kidney (Facts and dreams). Antiseptic63, 208–225 (1966)
Pettinger, W. A., Marchelle, M., Augusto, L.: Renin suppression by DOC and NaCl in the rat. Amer. J. Physiol.221, 1071–1074 (1971)
Regoli, D., Schaechtelin, G., Peters, G.: The renin activity of rat plasma. In: Progress in Nephrology, G. Peters and F. Roch-Ramel, Eds., pp. 335–336. Berlin-Heidelberg-New York: Springer 1969
Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963)
Riedel, B., Bucher, O.: Die Ultrastruktur des juxtaglomerulären Apparates des Meerschweinchens. Z. Zellforsch.79, 244–258 (1967)
Schaechtelin, G., Chométy, F., Regoli, D., Peters, G.: Dosage de l'activité réninique d'extraits tissulaires par incubation avec un substrat naturel purifié. Helv. physiol. pharmacol. Acta24, 89–105 (1966)
Schaechtelin, G., Riedel, B.: Renin-Aktivität in Nieren ohne “Renin-Granula”. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path.255, 65 only (1966)
Skelton, F. R., Chandra, S., Hubbard, J. C., Bernardis, L. L.: Studies on the genesis of the juxtaglomerular cell granule. Circulat. Res.20/21, Suppl. 1/2, 29–46 (1967)
Tobian, L., Thompson, Twedt, T., Janecek, J.: The granulation of juxtaglomerular cells in renal hypertension, desoxycorticosterone and postdesoxycorticosterone hypertension, adrenal regeneration hypertension, and adrenal insufficiency. J. clin. Invest.37, 660–671 (1958)
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Supported by a grant-in-aid of Fondation Fritz Hoffmann-La Roche pour l'expansion, en Suisse, du travail scientifique exécuté par équipe and by Fonds National Suisse de la Recherche Scientifique (Grants No. 5315.3 and 3.369.70).
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Riedel, B., Bucher, O., Peters-Haefeli, L. et al. The replenishment of the juxtaglomerular renin store after temporary renin depletion. Pflugers Arch. 347, 125–135 (1974). https://doi.org/10.1007/BF00592394
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DOI: https://doi.org/10.1007/BF00592394