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Dissociation between antidiuretic response and renal medullary cyclic AMP levels in the rat

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

In vivo experiments were performed in male Wistar rats to elucidate the probable relation between renal concentrating ability and medullary cyclic AMP content as influenced by changes of hydration and by administration of antidiuretic hormone (ADH). Cyclic AMP levels were 37% lower in water diuretic than in control animals (P<0.01), but were not significantly changed during prolonged antidiuresis induced by dehydration or ADH administration. Nor could any change of cyclic AMP levels be demonstrated between 2 and 20 min after ADH injection. Significant increases of medullary cyclic AMP content occurred following stress, anesthesia, and administration of isoproterenol and 3-isobutyl-1-methylxanthin. The results suggest that the level of cyclic AMP in the renal medulla may not be an important determinant of the antidiuretic response produced by ADH in rats.

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Abbreviations

cyclic AMP:

adenosine 3′,5′-cyclic monophosphate

ADH:

antidiuretic hormone

AVP:

arginine vasopressin

DDAVP:

1-deamino-8-d-arginine vasopressin

IBMX:

3-isobutyl-1-methylxanthin

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  1. Department of Pharmacology, University of Copenhagen, 20 Juliane Maries Vej, DK-2100, Copenhagen Ø, Denmark

    Sten Christensen

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  1. Sten Christensen
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Christensen, S. Dissociation between antidiuretic response and renal medullary cyclic AMP levels in the rat. Pflugers Arch. 374, 229–234 (1978). https://doi.org/10.1007/BF00585599

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  • DOI: https://doi.org/10.1007/BF00585599

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