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Topographical distribution of the secretin- and VIP-stimulated adenylate cyclase system in the heart of five animal species

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Adenylate cylase stimulation by secretin and VIP was compared to the effect of glucagon,d,l-isoproterenol, Gpp[[NH]p, and NaF in atria and ventricles from rat, guinea pig, rabbit, dog and Cynomolgus monkey. In rat ventricular membranes, secretin was a better stimulant than VIP and was as active asd,l-isoproterenol. In rat auricular membranes both peptides were inactive. In guinea pig and rabbit heart membranes (ventricular and auricular) VIP and secretin were inactive. In dog and monkey atria, VIP stimulation of adenylate cyclase was comparable to that ofd,l-isoproterenol, secretin being inactive. In dog ventricules, VIP was less efficient thand,l-isoproterenol, secretin being inactive. In monkey ventricles, by contrast, VIP was slightly more efficient thand,l-isoproterenol, secretin having a small effect only in left ventricles. The present results established a clear difference between animal species with respect to the efficacy of the peptides of the secretin/VIP family: the presence of “secretin-preferring” receptors in rat heart contrasted with the presence of “VIP-preferring” receptors in dog and monkey heart. Our results in dog and monkey hearts suggest that VIP might be a candidate for a physiological control of heart function.

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Abbreviations

VIP:

vasoactive intestinal peptide

Sn:

secretin

Gpp[NH]p:

guanosine 5′-O-(2-3-imido) triphosphate

EGTA:

ethylene glycol bis (2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

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Authors and Affiliations

  1. Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Boulevard de Waterloo 115, B-1000, Brussels, Belgium

    Patrick Robberecht, Magali Waelbroeck, Philippe De Neef, Jean-Claude Camus & Jean Christophe

  2. Continental Pharma Research Laboratories, B-1348, Mont-Saint-Guibert, Belgium

    Pierre Chatelain, Auh Nguyen Huu & Joseph Roba

Authors
  1. Pierre Chatelain
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  2. Patrick Robberecht
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  3. Magali Waelbroeck
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  4. Philippe De Neef
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  5. Jean-Claude Camus
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  6. Auh Nguyen Huu
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  7. Joseph Roba
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  8. Jean Christophe
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Chatelain, P., Robberecht, P., Waelbroeck, M. et al. Topographical distribution of the secretin- and VIP-stimulated adenylate cyclase system in the heart of five animal species. Pflugers Arch. 397, 100–105 (1983). https://doi.org/10.1007/BF00582046

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  • DOI: https://doi.org/10.1007/BF00582046

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