Abstract
Using double-barreled Cl−-sensitive microelectrodes, intracellular Cl− activity (A iCl ) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting stateA iCl was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (V m) of −41 mV. Addition of acetylcholine (ACh, 1μM) hyperpolarizedV m to −63 mV and decreasedA iCl to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes inV m andA iCl to those induced by ACh. It was concluded that Cl− was actively accumulated in the acinar cells and, in the secretory phase, Cl− efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl− permeability across the cell membrane.
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Saito, Y., Ozawa, T., Hayashi, H. et al. Acetylcholine-induced change in intracellular Cl− activity of the mouse lacrimal acinar cells. Pflugers Arch. 405, 108–111 (1985). https://doi.org/10.1007/BF00584530
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DOI: https://doi.org/10.1007/BF00584530