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Relationship between parvalbumin content and the speed of relaxation in chronically stimulated rabbit fast-twitch muscle

  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

The time courses of changes in parvalbumin (PA) content, isometric twitch tension, and half-relaxation time (1/2RT) were studied in rabbit tibialis anterior muscle following chronic 10 Hz nerve stimulation of 1–21 days. Up to 5 days stimulation had no effect on PA content, but it induced a slight (10–15%) increase in the 1/2RT. This change occurred together with the previously observed 50% decrease in Ca2+-uptake by the SR (Leberer et al. 1987). While prolonged stimulation produced no further decrease in the Ca2+-uptake by the SR, PA content declined after 5 days of stimulation. The reduction in PA content was accompanied by a progressive lengthening of the 1/2RT. However, the increase in 1/2RT was particularly pronounced after PA had fallen below 50% of its normal value. A 90% reduction in PA coincided with a 60% increase in the 1/2RT. By this time the staircase phenomenon, normally observed in fast-twitch muscle, was completely abolished. Although the changes in PA content and 1/2RT were not linearly related, these results suggest that PA plays an important role in the relaxation process of mammalian fast-twitch muscle.

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Author notes

  1. Gary A. Klug

    Present address: College of Human Development and Performance and Institute of Neuroscience, University of Oregon, 97403, Eugene, OR, USA

  2. Ekkehard Leberer

    Present address: Banting and Best Department of Medical Research Charles H. Best Institute, University of Toronto, 112 College Street, M5G 1L6, Toronto, Ontario, Canada

Authors and Affiliations

  1. Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750, Konstanz, Federal Republic of Germany

    Gary A. Klug, Ekkehard Leberer, Elmi Leisner, Jean-Aimé Simoneau & Dirk Pette

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  1. Gary A. Klug
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  4. Jean-Aimé Simoneau
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  5. Dirk Pette
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Klug, G.A., Leberer, E., Leisner, E. et al. Relationship between parvalbumin content and the speed of relaxation in chronically stimulated rabbit fast-twitch muscle. Pflugers Arch. 411, 126–131 (1988). https://doi.org/10.1007/BF00582304

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  • DOI: https://doi.org/10.1007/BF00582304

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