Abstract
(1) The effect of 30 μM D600 on the amplitude and time course of isometric contractures was studied in single slow fibres ofRana temporaria. (2) D600 only slightly reduced the amplitude of contractures evoked with 30 or 95 mM K-Ringer's. Maintenance of tension was strongly impaired by D600 only during exposure to 95 mM K. The caffeine contracture was not affected. (3) Addition of 10 mM Ca2+ or other divalent cations to the medium strongly counteracted the effect of D600 on maintained tension. The order of efficiency was Ca2+=Ni2+>Co2+>Mn2+>Mg2+. (4) During 2 min exposure to 95 mM K-Ringer's the slow fibres inactivated to a variable degree; recovery from inactivation in normal Ringer's proceeded with a half time of the order of 1 min, while in the presence of D600 recovery was prolonged 3.3 to 27 times. (5) It is concluded that the effect of D600 on the contractile behaviour of slow fibres fromRana temporaria is predominantly due to a prolongation of the inactivated state. It is suggested that D600 binds to a site at the outer membrane surface which also binds divalent cations and determines the degree of contractile inactivation during exposure to potassium. Blocking of Ca2+ channels is unlikely to be the mechanism of this D600-effect.
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References
Adrian H, Chandler WK, Rakowski RF (1976) Charge movement and mechanical repriming in skeletal muscle. J Physiol 254:361–388
Almers W, Palade PT (1981) Slow calcium and potassium currents across frog muscle membrane: Measurements with a vaselinegap technique. J Physiol 312:159–176
Almers W, Fink R, Palade PT (1981) Calcium depletion in frog muscle tubules: The decline of calcium current under maintained depolarization. J Physiol 312:177–207
Caputo C (1972) The time course of potassium contractures of single muscle fibres. J Physiol 223:483–505
Caputo C (1983) Pharmacological investigations of excitation-contraction coupling. Handbook of physiology, section 10, Skeletal muscle. Am Physiol Soc, Bethesda, Maryland 1983, pp 381–415
Caputo C, Bolaños P (1987) Contractile inactivation in frog skeletal muscle fibers. The effects of low calcium, tetracaine, dantrolene, D-600, and nifedipine. J Gen Physiol 89:421–442
Chandler WK, Rakowski RF, Schneider MF (1976) Effects of glycerol treatment and maintained depolarization on charge movment in skeletal muscle. J Physiol 254:285–316
Cota G, Siri IN, Stefani E (1984) Calcium channel inactivation in frog (Rana pipiens andRana moctezuma) skeletal muscle fibres. J Physiol 354:99–108
Eisenberg RS, McCarthy RT, Milton RL (1983) Paralysis of frog skeletal muscle fibres by the calcium antagonist D-600. J Physiol 341:495–505
Fabiato A, Fabiato F (1977) Calcium release from the sarcoplasmic reticulum. Circ Res 40:119–129
Fill MD, Best PM (1986) Contracture of skinned skeletal muscle fibres induced by ionic substitution: Comparison of block by D600 and nitrendipine. Biophys J 49:13a
Frankenhaeuser B, Lännergren J (1967) The effect of calcium on the mechanical response of single twitch muscle fibres of Xenopus laevis. Acta Physiol Scand 69:242–254
Gilly WF, Hui CS (1980a) Mechanical activation in slow and twitch skeletal muscle fibres of the frog. J Physiol 301:137–156
Gilly WF, Hui CS (1980b) Voltage-dependent charge movement in frog slow muscle fibres. J Physiol 301:175–190
Hodgkin AL, Horowicz P (1960) Potassium contractures in single muscle fibres. J Physiol 153:336–403
Huerta M, Stefani E (1981) Potassium and caffeine contractures in fast and slow muscles of the chicken. J Physiol 318:181–189
Huerta M, Muñiz J, Stefani E (1986) Effects of external calcium on potassium contractures in tonic muscle fibres of the frog (Rana pipiens). J Physiol 376:219–230
Hui CS, Milton RL, Eisenberg RS (1984) Charge movement in skeletal muscle fibers paralyzed by the calcium-entry blocker D600. Proc Natl Acad Sci USA 81:2582–2585
Kaumann AJ, Uchitel OD (1976) Reversible inhibition of potassium contractures by optical isomers of verapamil and D600 on slow muscle fibres of the frog. Naunyn-Schmiedeberg's Arch Pharmacol 292:21–27
Kikuchi T, Schmidt H (1983) Changes in resting and contractile properties of chicken muscles following denervation. Biomed Res 4(3):303–314
Kuffler SW, Vaughan Williams EM (1953) Properties of the ‘slow’ skeletal muscle fibres of the frog. J Physiol 121:318–340
Lännergren J (1967) The effect of calcium on potassium contractures of single slow muscle fibres of Xenopus laevis. Acta Physiol Scand 70:16–25
Lehmann N, Schmidt H (1979) Contractile responses to direct stimulation of frog slow muscle fibres before and after denervation. Pflügers Arch 382:43–50
Lüttgau HC (1963) The action of calcium ions on potassium contractures of single muscle fibres. J Physiol 168:679–697
McCleskey EW (1985) Calcium channels and intracellular calcium release are pharmacologically different in frog skeletal muscle. J Physiol 361:231–249
Miledi R, Parker I, Zhu PH (1981) Calcium transients in normal and denervated slow muscle fibres of the frog. J Physiol 318:191–206
Miledi R, Parker I, Zhu PH (1983) Calcium transients studied under voltage-clamp control in frog twitch muscle fibres. J Physiol 340:649–680
Nasledov GA, Zachar J, Zacharova D (1966) The ionic requirements for the development of contracture in isolated slow muscle fibres of the frog. Physiol Bohemoslov 15:293–306
Page SG (1969) Structure and some contractile properties of fast and slow muscles of the chicken. J Physiol 205:131–145
Pizarro G, Fitts R, Rios E (1988) Selectivity of a cation-binding membrane site essential for EC coupling in skeletal muscle. Biophys J 53:645a
Palade PT, Almers W (1985) Slow calcium and potassium currents in frog skeletal muscle: their relationship and pharmacologic properties. Pflügers Arch 405:91–101
Rios E, Brum G (1987) Involvement of dihydropyridine receptors in excitation-contraction coupling in skeletal muscle. Nature 325:717–720
Sanchez JA, Stefani E (1978) Inward calcium current in twitch muscle fibres of the frog. J Physiol 283:197–209
Schmidt H (1987) Effect of divalent cations on the potassium contracture of slow muscle fibres ofRana temporaria. J Membr Biol 100:215–220
Schmidt H, Siebler M (1985) Persistance of excitation-contraction coupling (ECC) in normal and denervated slow fibres treated with D600. Pflügers Arch 405 (Suppl):R59
Schmidt H, Siebler M (1986) Effects of D600 on single slow fibres ofRana temporaria. Pflügers Arch 406 (Suppl.):R30
Siebler M, Schmidt H (1987) D600 prolongs inactivation of the contractile system in frog twitch fibres. Pflügers Arch 410: 75–82
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This work was supported by the Deutsche Forschungsgemeinschaft (SFB 38 and 246), Bonn-Bad Godesberg. Thanks are due to B. Jung and M. Sinnwell for technical and secretarial help
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Schmidt, H., Siebler, M. & Krippeit-Drews, P. The effect of D600 on potassium contractures of slow muscle fibres ofRana temporaria . Pflugers Arch. 412, 390–396 (1988). https://doi.org/10.1007/BF01907557
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DOI: https://doi.org/10.1007/BF01907557