Abstract
The effect of antidiuretic hormone (ADH) on transepithelial Na+, Cl−, Ca2+ and Mg2+ net fluxes (JNa, JCl, JMg, JCa) was investigated in isolated perfused cortical thick ascending limb segments (cTAL) of the mouse nephron, using the microperfusion technique and the electron microprobe analysis to determine the ionic composition of the collected tubular fluid. Simultaneously, the transepithelial potential difference (PDte) and the transepithelial resistance (Rte) were recorded. Prior to the flux measurements cTAL segments were perfused for one hour. During this equilibration period PDte decreased significantly from +19.9±1.6 to +14.9±1.l mV and Rte increased from 30.6±3.5 Ωcm2 to 38.8±2.4 Ωcm2 (n=7), reflecting a decline in NaCl transport. After ADH was added to the bath solution at 10−10 mol.l−1, PDte increased from +14.4±1.1 to +18.0±1.5 mV, accompanied by a rise in JNa and JCl from 205±11 to 273±19 and from 216±12 to 283±21 pmol.min−1.mm−1 (n=7), respectively. JCa and JMg also increased from 0.81±0.07 to 1.50±0.12 and from 0.43±0.11 to 0.76±0.08 pmol.min−1.mm−1 (n=7), respectively. All these effects were fully reversible after withdrawal of the hormone. In conclusion our data indicate that ADH stimulates divalent cation transport and NaCl transport in the cortical thick ascending limb of Henle's loop of the mouse.
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References
Burg MB, Grantham J, Abramov M, Orloff J (1966) Preparation and study of fragments of single rabbit nephrons. Am J Physiol 219:1293–1298
Di Stefano A, Wittner M, Greger R (1988) Bicarbonate is not required for NaCl reabsorption in the cortical thick ascending limb of mouse nephron. Acta Physiol Pharmacol Bulgarica 14 (Suppl 1):32 (abstract)
Greger R (1981) Cation selectivity of the isolated perfused cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 390:30–37
Greger R, Schlatter E (1983) Properties of the basolateral membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch. 396:325–334
Hebert SC, Culpepper M, Andreoli TE (1981) NaCl transport in mouse medullary thick ascending limbs. I.Functional heterogeneity and ADH-stimulated NaCl transport. Am J Physiol 241:F412-F431
Morel F, Roinel N (1966) Application de la microsonde électronique à l'analyse élémentaire quantitative d'échantillons biologiques liquides d'un volume inférieur à 10−9 mol.l−1. J Chim Physiol Chim Biol 66:1084–1091
Morel F, Imbert-Teboul M, Charbadès D (1981) Distribution of hormone-dependent adenylate cyclase in the nephron and its physiological significance. Annu Rev Physiol 43: 569–581.
Wittner M, Di Stefano A, Wangemann P, Nitschke R, Greger R, Bailly C, Amiel C, Roinel N, de Rouffignac C (1988) Differential effects of ADH on sodium, chloride, potassium, calcium and magnesium transport in cortical and medullary thick ascending limbs of mouse nephron. Pflügers Arch. 412:516–523
Wittner M, Di Stefano A (1990) Effects of antidiuretic hormone, parathyroid hormone and glucagon on transepithelial voltage and resistance of the cortical and medullary thick ascending limb of Henle's loop of the mouse nephron. Pflügers Arch. 415:707–712
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Wittner, M., Di Stefano, A., Mandon, B. et al. Stimulation of NaCl reabsorption by antidiuretic hormone in the cortical thick ascending limb of Henle's loop of the mouse. Pflügers Arch. 419, 212–214 (1991). https://doi.org/10.1007/BF00373010
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DOI: https://doi.org/10.1007/BF00373010