Heterooligomeric assembly of inward-rectifier K⁺ channels from subunits of different subfamilies: K_ir2.1 (IRK1) and K_ir4.1 (BIR10)

Abstract

 Activities of strong inward-rectifier K⁺ channels composed of K_ir2.1(84 M), K_ir2.1(84T) and K_ir4.1 subunits and weak inward-rectifier K⁺ channels composed of K_ir4.1(E158N) subunits were measured from giant inside-out patches of Xenopus laevis oocytes. The conductance/voltage (g/V) relationship for block by intracellular spermine (SPM) was biphasic for both K_ir2.1 channel types while it was monophasic for both K_ir4.1 channel types. The release of blocking Mg²⁺ ions was slow for K_ir2.1(84T) but virtually instantaneous for K_ir2.1(84M) and both K_ir4.1 channel types. Coexpression of K_ir2.1(84T) and K_ir4.1(E158N) resulted in heterooligomeric channels which were strongly rectifying, with a g/V relationship for SPM-evoked block that was significantly different from that of either parental homooligomeric channel type. Block by intracellular Mg²⁺ was markedly stronger than that for K_ir4.1(E158N) channels, while release of the block was almost instantaneous, similar to that for K_ir4.1(E158N) channels. This suggests preferential formation of a particular heterooligomer such as was recently proposed for subunits within the K_ir3.0 family.