Summary
Reabsorption rates for urate in the loops of Henle were measured in superficial nephrons in the rat 1. under conditions of free flow, 2. using microperfusion and 3. by a microinjection technique. 1. Under conditions of free flow distally measured TF/PUA/TF/PIn-values varied between 0.51 and 0.38 in antidiuretic rats, depending on TF/PIn (UA = both uric acid and urate, In = inulin, TF/P = concentration in tubular fluid to plasma concentration). The corresponding values in samples from end-proximal tubules were 1.06 and in urine 0.19 (U/PUA/U/PIn). 2. In microperfusion experiments of Henle loops early distal recoveries of 2-C14 urate varied between 57 and 86%, depending on the flow rates (10–40 nl/min). 3. In microinjection experiments C14 recovery in urine was about 85% when tracer solution was microinjected into endproximal tubules.
From these results we conclude: 1. The main site of urate reabsorption is located in the loops of Henle. 2. This reabsorption is highly dependent on flow rates. Increase of flow rate through Henle's loop decreases urate reabsorption.
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Fischer, V., Heidland, A., Klütsch, K., Gekle, D.: Phosphat- und Harnsäuretransport bei terminaler Niereninsuffizienz. Verh. dtsch. Ges. inn. Med.74, 1064–1068 (1968)
Greger, R., Lang, F., Deetjen, P.: Handling of uric acid by the rat kidney. I. Microanalysis of uric acid in proximal tubular fluid. Pflügers Arch.324, 279–287 (1971)
Kessler, R. H., Hierholzer, K., Gurd, R. S.: Localisation of urate transport in the nephron of mongrel and Dalmatian dog kidney. Amer. J. Physiol.197, 601–603 (1959)
Kramp, R. A., Lassiter, W. E., Gottschalk, C. W.: Urate-2-14C transport in the rat nephron. J. clin. Invest.50, 35–48 (1971)
Lang, F., Greger, R., Deetjen, P.: Handling of uric acid by the rat kidney. III. Microperfusion studies on steady state concentration of uric acid in the proximal tubule. Pflügers Arch.338, 295–302 (1973)
Morel, F., Mylle, M., Gottschalk, C. W.: Tracer microinjection studies of the effect of ADH on renal tubular diffusion of water. Amer. J. Physiol.209, 179–187 (1965)
Mudge, G. H., McAlary, B., Berndt, W. O.: Renal transport of uric acid in the guinea pig. Amer. J. Physiol.214, 875–879 (1968)
Oelert, H., Baumann, K., Gekle D.: Permeabilitätsmessungen einiger schwacher organischer Säuren aus dem distalen Konvolut der Rattenniere. Pflügers Arch.307, 178–189 (1969)
Schnermann, J., Davis, J. M., Wunderlich, P., Levine, D. Z., Horster, M.: Technical problems in the micropuncture determination of nephron filtration rate and their functional implications. Pflügers Arch.329, 307–320 (1971)
Skeith, M. P., Healy, L. A., Cutler, R. E.: Urate excretion during mannitol diuresis. J. Lab. clin. Med.70, 213–220 (1967)
Steele, T. H., Rieselbach, R. E.: The renal mechanism for urate homeostasis in normal man. The contribution of residual nephrons within the chronically diseased kidney to urate homeostasis in man. Amer. J. Med.43, 868–886 (1967)
Steele, T. H., Underwood, J. L., Dudgeon, K. L.: Urate excretion and urine flow in a lithium-induced diabetes insipidus rat model. Pflügers Arch.346, 205–213 (1974)
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Partially supported by the “Österreichischer Fond zur Förderung der wissenschaftlichen Forschung”.
Part of this work was presented at the VII. Symp. Ges. Nephr. (1970), Tübingen.
Receiving scholarships from the “Deutsche Forschungsgemeinschaft”.
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Greger, R., Lang, F. & Deetjen, P. Urate handling by the rat kidney. Pflugers Arch. 352, 115–120 (1974). https://doi.org/10.1007/BF00587510
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DOI: https://doi.org/10.1007/BF00587510