Abstract
We have analyzed the design and operation of hollow fiber bioreactors for cell culture on the basis of cell growth efficiency and non-uniform fiber spacing. Operating diagrams are presented that describe reactor performance as a function of important operating variables like oxygen concentration and the fiber packing density. The diagrams allow one to find the best set of operating conditions for a fixed reactor design, or to rationally adjust the design parameters for fixed operating conditions.
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Abbreviations
- C in :
-
feed oxygen concentration (mass/volume)
- D 2 :
-
diffusivity of oxygen in continuous cell mass (length/time)
- R 2 :
-
outer radius of hollow fiber (length)
- ζ :
-
packing efficiency (dimensionless)
- φ:
-
Thiele modulus (dimensionless)
- Φ:
-
cellular oxygen consumption rate (mass/volume-time)
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Sardonini, C., DiBiasio, D. Design and operating criteria for hollow fiber bioreactors. Bioprocess Engineering 15, 327–330 (1996). https://doi.org/10.1007/BF02426443
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DOI: https://doi.org/10.1007/BF02426443