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Precipitation of protein by ultracentrifuge with angle rotor

Part 2: Experimental

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Abstract

Precipitation of a protein by ultracentrifuge with an angle rotor was investigated experimentally. Experimental data for Lysozyme and Bovine serum albumin were compared with the calculated distribution of concentration, and an agreement between calculated curves and experimental data during the course of ultracentrifugation was obtained. From the first approximated model presented in our previous paper, it is observed that the precipitation of a protein by ultracentrifuge with an angle rotor proceeds more rapidly than that with a swing rotor.

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Abbreviations

c a kg/m3 :

concentration of solute for angle rotor

D cm2/s:

diffusion coefficient

d cm:

diameter of ultracentrifugal tube

r * :

dimensionless radial coordinate

r 1 cm:

minimum radius of ultracentrifugal tube

r 2 cm:

maximum radius of ultracentrifugal tube

s s:

sedimentation constant

t s:

time

α :

dimensionless parameter

θ deg:

inclination of ultracentrifugal tube

ω s−1 :

angular velocity of rotation

References

  1. Charlwood, P. A.: End effects in centrifuge tubes in rate-zonal and isopycnic experiments. J. Phys. Chem. 84 (1980) 3122–3125

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  2. Shiragami, N.; Kajiuchi, T.: Precipitation of protein by ultracentrifuge with angle rotor. Part 1: Model for sedimentation process. Bioprocess Eng. (1990) 85–88

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Authors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, 2-12-1 Oookayama, Meguro-ku, Tokyo

    N. Shiragami & A. Matsuda

  2. Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Japan

    T. Kajiuchi

Authors
  1. N. Shiragami
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  2. T. Kajiuchi
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  3. A. Matsuda
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Additional information

Part 1 of this article was published in Vol. 5. No. 2

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Shiragami, N., Kajiuchi, T. & Matsuda, A. Precipitation of protein by ultracentrifuge with angle rotor. Bioprocess Engineering 5, 103–105 (1990). https://doi.org/10.1007/BF00388187

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  • DOI: https://doi.org/10.1007/BF00388187

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