Abstract
Cultivation of Brevibacterium divaricatum for glutamic acid production in an airlift reactor with net draft tube was developed. Cell concentration gave an index for adding penicillin G. On-line estimation of total sugar concentration yielded an identified model which was used for determination of the substrate addition. Fermentation for glutamic acid production requires high oxygen concentration in the broth. The proposed reactor has the capability to provide sufficient oxygen for the fermentation. Since the reactor is suitable for fed-batch culture, the cultivation of B. divaricatum for glutamic acid production in the proposed reactor is successfully carried out.
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Abbreviations
- a :
-
system parameter
- b :
-
system parameter
- C c,in :
-
mole fraction carbon dioxide in the gas inlet
- C c,out :
-
mole fraction carbon dioxide in the gas outlet
- C L mole/dm3 :
-
oxygen concentration in liquid phase
- C * L mole/dm3 :
-
saturated oxygen concentration in liquid phase
- C 0,in :
-
mole fraction of oxygen in the gas inlet
- C 0,out :
-
mole fraction of oxygen in the gas outlet
- CPR mole/h/dm3 :
-
carbon dioxide production rate based on total broth
- E(t) :
-
error signal
- F in mole/h:
-
inlet gas flow rate
- k 1 :
-
constant defined by Eq. (4)
- k 2 :
-
constant defined by Eq. (5)
- k L a 1/h:
-
volumetric mass transfer coefficient of gas-liquid phase
- OUR mole/h/dm3 :
-
oxygen uptake rate based on total broth
- P atm:
-
pressure in the reactor
- t h:
-
time
- TS c g:
-
total sugar consumption
- TS s g/dm3 :
-
set point of total sugar concentration
- TS * g/dm3 :
-
reference value of total sugar concentration
- TS(t) g/dm3 :
-
total sugar concentration in the broth at timet
- u(t) cm3/min:
-
feed rate at timet
- V dm3 :
-
total broth volume
- VVM (dm3/min)/dm3 :
-
flow rate per unit liquid volume
- Λ :
-
a negative constant defined by Eq. (7)
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Wu, J.Y., Wu, W.T. Glutamic acid production in an airlift reactor with net draft tube. Bioprocess Engineering 8, 183–187 (1992). https://doi.org/10.1007/BF01254235
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DOI: https://doi.org/10.1007/BF01254235