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Enzyme sensor for L-lactate with a chitosan-mercury film electrode

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Summary

An amperometric enzyme sensor composed of a mercury film electrode and an enzyme-immobilized chitosan membrane is developed. This biosensor is based on both a mercury film electrode detecting the consumption of dissolved dioxygen following enzymatic reaction, and a chitosan membrane. The latter provides an excellent permselectivity and excludes electroactive interferents. The detection range of this biosensor was 1.0×10−5–3.0×10−4 mol/l and the relative standard deviation, R.S.D. at 5.0×10−5 mol/l was 1.4% (n=3). This biosensor was applied to the direct determination of L-lactate in human serum without pretreatment.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hokkaido University, 060, Sapporo, Japan

    Satoshi Hikima, Teiji Kakizaki, Mitsuhiko Taga & Kiyoshi Hasebe

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  1. Satoshi Hikima
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  2. Teiji Kakizaki
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  3. Mitsuhiko Taga
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  4. Kiyoshi Hasebe
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Hikima, S., Kakizaki, T., Taga, M. et al. Enzyme sensor for L-lactate with a chitosan-mercury film electrode. Fresenius J Anal Chem 345, 607–609 (1993). https://doi.org/10.1007/BF00325809

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  • DOI: https://doi.org/10.1007/BF00325809

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