Skip to main content
SpringerLink
Log in

Maturation postnatale du tractus optique chez le Rat: Identification et quantification de la névroglie sur radio-autographies

Postnatal maturation of the optic tract in the Rat: Identification and quantifying of the neuroglia on radio-autographs

  • Published:
Experimental Brain Research Aims and scope Submit manuscript

Summary

A study based on the histological identification and quantifying of the neuroglia cells, was carried out in the optic tract of the Rat on radioautographs after injection of tritiated thymidine. The assessment was made two and a half days and twenty days after birth. Qualitatively speaking, three cellular categories can be distinguished: the oligodendrocytes, themselves divided into three sorts (big, medium., small) the astrocytes and other cellular varieties including the microglia. Quantitatively speaking the oligodendrocytes, in particular the small ones, are decidedly more numerous. In fact, over the whole period under consideration the oligodendrocytes represent nearly 81% of the total population. From the point of view of the radio-autography the labeling index predominates in the medium oligodendrocyte group. These results are discussed with reference in particular to the myelination process which is preceded directly by the phenomena of gliosis.

Resume

Une étude, basée sur l'identification et la quantification histologique des cellules névrogliques, a été entreprise dans le tractus optique du Rat sur radio-autographies après injection de thymidine tritiée. Ce bilan a été effectué entre 2 jours et demi et 20 jours après la naissance. D'un point de vue qualitatif, trois catégories cellulaires peuvent être distinguées: les oligodendrocytes, euxmêmes subdivisés en trois classes (grands, moyens, petits), les astrocytes et d'autres varieties cellulaires parmi lesquelles la microglie. D'un point de vue quantitatif, les oligodendrocytes, en particulier les petits, sont largement majoritaires. En effet, sur toute la période examinée les oligodendrocytes représentent près de 81% de la population totale. Sur le plan radio-autographique, l'index de marquage prédomine dans la classe des moyens oligodendrocytes. Ces résultats sont discutés, notamment par rapport aux processus de myélinisation dont les phénomènes de gliose constituent le préambule direct.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abercrombie, M.: Estimation of nuclear population from microtome sections. Anat. Rec. 94, 239–247 (1946).

    Article  CAS  PubMed  Google Scholar 

  • Altman, J.: Autoradiographic study of degenerative and regenerative proliferation of neuroglia cells with tritiated thymidine. Exp. Neurol. 5, 302–318 (1962).

    Article  CAS  PubMed  Google Scholar 

  • —: Proliferation and migration of indifferentiated precursor cells in the rat during postnatal gliogenesis. Exp. Neurol. 16, 263–278 (1966).

    Article  CAS  PubMed  Google Scholar 

  • Asbury, A.K.: Schwann cell proliferation in developing mouse sciatic nerve. A radio-autographic study. Cell. Biol. 34, 735–743 (1967).

    Article  CAS  Google Scholar 

  • Blenk, H.: Quantitative Untersuchungen an Gliazellen im Corpus callosum wachsender Katzen nach Perfusions-und Immersionsfixierung. Z. Anat. Entwickl.-Gesch. 128, 222–234 (1969).

    Article  CAS  Google Scholar 

  • Blunt, M.J., Paisley, P.B., Wendell-Smith, C.P.: Oxidative enzyme histochemistry of immature neuroglia during myelination. J. Anat. (Lond.) 110, 421–433 (1971).

    CAS  Google Scholar 

  • Bunge, R.P.: Glial cells and the central myelin sheath. Physiol. Rev. 48, 197–251 (1968).

    CAS  PubMed  Google Scholar 

  • Caley, D.W., Maxwell, D.S.: An electron microscopic study of the neuroglia during postnatal development of the rat cerebrum. J. comp. Neurol. 133, 45–70 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Cammermeyer, J.: Morphologic distinctions between oligodendrocytes and microglia cells in the rabbit cerebral cortex. Amer. J. Anat. 118, 227–247 (1966a).

    Article  CAS  PubMed  Google Scholar 

  • —: The association between microglia and myelin in silver-impregnated sections. Z. Anat. Entwickl.-Gesch. 125, 367–377 (1966b).

    Article  Google Scholar 

  • Collewijn, H., Schadé, J.P.: Changes in the size of astrocytes and oligodendrocytes during anoxia hypothermia and spreading depression. In: Biology of neuroglia. Progr. Brain Res. 15, 184–195 (1965).

    Article  Google Scholar 

  • Fleischhauer, K.: Über die Reihenhildung der Gliazellen in der weißen Substanz. Anat. Anz. 121, Suppl., 433–435 (1968a).

    Google Scholar 

  • —: Postnatale Entwicklung der Neuroglia. Acta neuropath. (Berl.) Suppl. IV, 20–32 (1968b).

    Google Scholar 

  • Friede, R.L.: Glia and myelination. VIè Cong. In: Intern. Neuropath., pp. 293–305. Paris: Masson et Cie 1970.

    Google Scholar 

  • Fulcrand, J., Bisconte, J., Marty, R.: Radioautographie quantitative de la prolifération névroglique au cours du développement postnatal chez le Rat. Z. mikr.-anat. Forsch. 82, 349–361 (1970a).

    CAS  PubMed  Google Scholar 

  • —, Marty, R.: Prolifération névroglique postnatale chez le Rat: radioautographie quantitative. VIè Cong. In: Intern. Neuropath., pp. 445–446. Paris: Masson et Cie 1970b.

    Google Scholar 

  • —: Migrations névrogliques dans le tractus optique chez le Rat: radio-autographie quantitative au cours du développement postnatal. Exp. Brain Res. 14, 38–47 (1971).

    Article  CAS  PubMed  Google Scholar 

  • lees, P., Meller, K.: Morphology of neuroglia. In: Bourne I: Structure and function of nervous tissue, pp. 301–323 (1968).

  • Hirano, A.: A confirmation of the oligodendroglial origin of myelin in the adult rat. J. Cell Biol. 38, 637–640 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hommes, O., Leblond, C.P.: Mitotic division of neuroglia in the normal adult rat. J. comp. Neurol. 129, 269–278 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Inoue, Y.: The glioarchitectonics of the chicken brain. I. The glial cells in the optic tract. II. Microglia. Okajimas Folia anat. jap. 47, 229–265 (1970); 48, 53–82 (1971).

    Article  CAS  Google Scholar 

  • King, J.S.: A light and electron microscopic study of perineuronal glial cells and processus in the rabbit neoeortex. Anat. Rec. 161, 111–123 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Matthews, M.A., Duncan, D.: A quantitative study of morphological changes accompanying the initiation and progress of myelin production in the dorsal funiculus of the rat spinal cord. J. comp. Neurol. 142, 1–22 (1971).

    Article  CAS  PubMed  Google Scholar 

  • Maxwell, D.S., Kruger, L.: The reactive oligodendrocyte. An electron microscopic study of cerebral cortex following alpha particle irradiation. Amer. J. Anat. 118, 437–460 (1966).

    Article  CAS  PubMed  Google Scholar 

  • Mitrová, E.: Karyometric investigation of glia cells in the cerebellum in the course of myelination. Z. mikr.-anat. Forsch. 77, 304–312 (1967).

    PubMed  Google Scholar 

  • Moliner, E.R.: A study on neuroglia. The problem of transitional formes. J. comp. Neurol. 110, 157–171 (1958).

    Article  Google Scholar 

  • Mori, S., Leblond, C.P.: Identification of microglia in light and electron microscopy. J. comp. Neurol. 135, 57–80 (1969a).

    Article  CAS  PubMed  Google Scholar 

  • —: Electron microscopic features and proliferation of astrocytes in the corpus callosum of the rat. J. comp. Neurol. 137, 197–226 (1969b).

    Article  CAS  PubMed  Google Scholar 

  • —: Electron microscopic identification of three classes of oligodendrocytes and a preliminary study of their proliferative activity in the corpus callosum of young rats. J. comp. Neurol. 139, 1–30 (1970).

    Article  CAS  PubMed  Google Scholar 

  • Mugnaini, E., Walberg, F.: Ultrastructural of neuroglia. Ergebn. Anat. Entwickl.-Gesch. 37, 194–236 (1984).

    Google Scholar 

  • Noetzel, H., Siepmann, P.: Autoradiographische Untersuchungen am Großhirn neugeborener Ratten nach Injektion von Thymidine H3. Ein Beitrag zur Gliaentwicklung. Dtsch. Z. Nervenheilk. 187, 637–659 (1965).

    Article  Google Scholar 

  • Privat, A.: Sur l'origine des divers types de névroglie chez le rat. VIè Cong. In: Intern. Neuropath., pp. 447–448. Paris: Masson et Cie 1970.

    Google Scholar 

  • Sjöstrand, J.: Proliferative changes in glial cells during nerve regeneration. Z. Zellforsch. 68, 481–493 (1965).

    Article  PubMed  Google Scholar 

  • —: Morphological changes in glial cells during nerve regeneration. Acta physiol. scand. Suppl. 270, 67, 19–43 (1966).

    Google Scholar 

  • Skoff, R.P., Vaughn, J.E.: An autoradiographic study of cellular proliferation in degenerating rat optic nerve. J. comp. Neurol. 141, 133–156 (1971).

    Article  CAS  PubMed  Google Scholar 

  • Smart, I., Leblond, C.P.: Evidence for division and transformations of neuroglia cells in the mouse brain, as derived from radioautography after injection of thymidine H3. J. comp. Neurol. 116, 349–367 (1961).

    Article  Google Scholar 

  • Stensaas, J., Stensaas, S.S.: Astrocytic neuroglial cells, oligodendrocytes and microgliacytes in the spinal cord of the toad I & II Light and electron microscopy. Z. Zellforsch. 84, 473–489, 86, 184–213 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Trumpy, J.H.: Transneuronal degeneration in the pontine nuclei of the cat. I. Neuronal changes in animals of varying ages. II. The glial proliferation. Ergehn. Anat. Entwickl.Gesch. 44, 7–46, 47–70 (1971).

    Google Scholar 

  • Vaughn, J.E., Peters, A.: A third nenroglial cell type an electron microscopic study. J. comp. Neurol. 133, 269–288 (1968).

    Article  CAS  PubMed  Google Scholar 

  • —: An electron microscopic analysis of gliogenesis in rat optic nerves. Z. Zellforsch. 94, 293–324 (1969).

    Article  CAS  PubMed  Google Scholar 

  • Wendell-Smith, C.P., Blunt, M.J., Baldwin, F.: The ultrastructural characterization of macroglial cell types. J. comp. Neurol. 127, 219–239 (1966).

    Article  CAS  PubMed  Google Scholar 

  • Wender, M., Kozik, M., Owsianowski, M.: A histoenzymatic study of neuroglia during myelination of the rabbit brain. Folia histochem. cytochem. 7, 135–150 (1969).

    CAS  Google Scholar 

  • Wolff, J.R.: Quantitative aspects of astroglia. VIè Cong. In: Intern. Neuropath., pp. 327–337. Paris: Masson et Cie 1970.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Neurophysiologie, Equipe de Recherche Associée au C. N. R. S., Université des Sciences et Techniques du Languedoc, Montpellier, France

    Jean Fulcrand & Robert Marty

Authors
  1. Jean Fulcrand
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert Marty
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fulcrand, J., Marty, R. Maturation postnatale du tractus optique chez le Rat: Identification et quantification de la névroglie sur radio-autographies. Exp Brain Res 16, 466–475 (1973). https://doi.org/10.1007/BF00234473

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00234473

Key words

Search

Navigation