Summary
Field and intracellular potentials were recorded in the vestibular nuclei of the frog following stimulation of the anterior branch of the ipsilateral vestibular nerve and the spinal cord. The field potential induced by stimulation of the vestibular nerve consisted of an early positive-negative wave followed by a slow negativity and that recorded during spinal cord stimulation was composed of an antidromic potential followed by a slow negative wave. These potentials were most prominent in the ventral region of the stato-acoustic complex. Mono- and polysynaptic EPSPs were recorded from vestibular neurons following vestibular nerve stimulation. Short latency depolarizations of small amplitude preceded the monosynaptic EPSPs in some neurons. Spike-like partial responses were commonly superimposed on the EPSPs. These all-or-none depolarizations probably originated in the dendrites. In a group of vestibular neurons stimulation of the vestibular nerve evoked full action potentials with latencies ranging from 0.2 to 1.1 msec. They are presumably caused by antidromic activation of neurons which send their axons to the labyrinth. The presence of efferent neurons in the vestibular nuclei was confirmed by their successful staining with Procion Yellow following axonal electrophoresis.
After stimulation of the spinal cord, antidromic spike potentials and EPSPs were recorded in vestibular neurons. In addition, short-latency depolarizing potentials (EDPs) were evoked by spinal stimulation, with latencies similar to those of antidromic potentials. The EDPs are suggested to be induced by electrotonic transmission from the neighboring cell and likely to be active spike potentials produced at some distance away from the soma.
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References
Baker, R., Llinás, R.: Electrotonic coupling between neurones in the rat mesencephalic nucleus. J. Physiol. (Lond.) 212, 45–63 (1971)
Bennett, M.V.L., Kriebel, M.E.: Neural control of electric organ discharge in the stargazer, Astroscopus: An example of oculomotor activity synchronized by electrotonic coupling. Fed. Proc. 27, 451 (1968)
Brookhart, J.M., Fadiga, E.: Potential fields initiated during monosynaptic activation of frog motoneurones. J. Physiol. (Lond.) 150, 633–655 (1960)
Burlet, H.M. de: Zur vergleichenden Anatomie der Labyrinthinnervation. J. comp. Neurol. 47, 155–169 (1929)
Corvaja, N., Grofová, I.: Vestibulospinal projection in the toad. In: Basic Aspects of Central Vestibular Mechanisms. Ed. by A. Brodal and O. Pompeiano. Progr. Brain Ees. 37, 297–307 (1972)
Eccles, J.C., Libet, B., Young, R.R.: The behavior of chromatolysed motoneurones studied by intracellular recordings. J. Physiol. (Lond.) 143, 11–40 (1958)
Gregory, K.M.: Central projections of the eighth nerve in the frog. Brain Behav. Evol. 5, 70–88 (1972)
Grinnell, A.D.: A study of the interaction between motoneurones in the frog spinal cord. J. Physiol. (Lond.) 182, 612–648 (1966)
Hillman, D.E.: Light and electron microscopical study of the relationships between the cerebellum and the vestibular organ of the frog. Exp. Brain Res. 9, 1–15 (1969)
Horcholle-Bossavit, G., Tyć-Dumont, S.: Evidence for a rapid transmission in the cat vestibuloocular pathway. Exp. Brain Res. 13, 327–338 (1971)
Iles, J.F., Mulloney, B.: Procion Yellow staining of cockroach motor neurons without the use of microelectrodes. Brain Res. 30, 397–400 (1971)
Ito, M., Hongo, T., Okada, Y.: Vestibular-evoked postsynaptic potentials in Deiters neurons. Exp. Brain Res. 7, 214–230 (1969)
Ito, M., Hongo, T., Yoshida, M., Okada, Y., Obata, K.: Antidromic and transsynaptic activation of Deiters' neurons induced from the spinal cord. Jap. J. Physiol. 14, 638–658 (1964)
Kidokoro, Y.: Cerebellar and vestibular control of fish oculomotor neurones. In: Neurobiology of Cerebellar Evolution and Development. Ed. by R. Llinás, pp. 257–276. Chicago: Amer. Med. Ass. Educ. and Res. Fed. 1969
Korn, H., Bennett, M.V.L.: Electrotonic coupling between teleost oculomotor neurons; restriction to somatic regions and relation to function of somatic and dendritic sites of impulse initiation. Brain Res. 38, 433–439 (1972)
Korn, H., Sotelo, C., Crepel, F.: Electrotonic coupling between neurons in the rat lateral vestibular nucleus. Exp. Brain Res. 16, 255–275 (1973)
Kriebel, M.E., Waxman, S.G., Pappas, G.D., Bennett, M.V.L.: Neurophysiology of oculomotor neurons in teleosts. Biol. Bull. 135, 426 (1968)
Kuno, M., Llinás, R.: Enhancement of synaptic transmission by dendritic potentials in chromatolysed motoneurones of the cat. J. Physiol. (Lond.) 210, 807–821 (1970)
Larsell, O.: The cerebellum of the frog. J. comp. Neurol. 36, 89–112 (1923)
Larsell, O.: The development of the cerebellum in the frog (Hyla regilla) in relation to the vestibular and lateral line system. J. comp. Neurol. 39, 249–289 (1925)
Ledoux, A.: Les canaux semi-circulaires. Etude électrophysiologique. Contribution a l'effort d'uniformisation des éprecevés vestibulaires. Essai d'interprétation de la semiologie vestibulaire. Acta oto-rhino-laryng. belg. 12, 109–348 (1958)
Llinás, R., Nicholson, C.: Electrophysiological properties of dendrites and somata in alligator Purkinje cells. J. Neurophysiol. 34, 532–551 (1971)
Llinás, R., Precht, W.: The inhibitory vestibular efferent system and its relation to the cerebellum in the frog. Exp. Brain Res. 9, 16–29 (1969)
Llinás, R., Precht, W., Clarke, M.: Cerebellar Purkinje cell responses to physiological stimulation of the vestibular system in the frog. Exp. Brain Res. 13, 408–431 (1971)
Mehler, W.R.: Comparative anatomy of the vestibular nuclear complex in submammalian vertebrates. In: Basic Aspects of Central Vestibular Mechanisms. Ed. by A. Brodal and O. Pompeiano. Progr. Brain Res. 37, 55–67 (1972)
Ozawa, S., Precht, W., Shimazu, H.: Crossed effects on central vestibular neurons in the horizontal canal system of the frog. Exp. Brain Res. 19, 394–405 (1974)
Precht, W., Grippo, J., Wagner, A.: Contribution of different types of central vestibular neurons to the vestibulo-spinal system. Brain Res. 4, 119–123 (1967)
Precht, W., Llinás, R.: Functional organization of the vestibular afferents to the Cerebellar cortex of frog and cat. Exp. Brain Res. 9, 30–52 (1969a)
Precht, W., Llinás, R.: Comparative aspects of the vestibular inputs to the cerebellum. In: Neurobiology of Cerebellar Evolution and Development. Ed. by R. Llinás, pp. 677–702. Chicago: Amer. Med. Ass. Educ. and Res. Fed. 1969b
Precht, W., Llinás, R., Clarke, M.: Physiological responses of frog vestibular fibers to horizontal angular rotation. Exp. Brain Res. 13, 378–407 (1971)
Precht, W., Shimazu, H.: Functional connections of tonic and kinetic vestibular neurons with primary vestibular afferents. J. Neurophysiol. 28, 1014–1028 (1965)
Purpura, D.P.: Comparative physiology of dendrites. In: The Neurosciences; A study program. Ed. by G.C. Quarton, T. Melnechuk and F.O. Schmitt, pp. 372–393. New York: Rockefeller Univ. Press 1967
Schmidt, R.S.: Frog labyrinthine efferent impulses. Acta oto-laryng. (Stockh.) 56, 51–64 (1963)
Spencer, W.A., Kandel, E.R.: Electrophysiology of hippocampal neurons. IV. Fast prepotentials. J. Neurophysiol. 24, 272–285 (1961)
Thomas, R.C., Wilson, V.J.: Precise localization of Renshaw cells with a new marking technique. Nature (Lond.) 206, 211–213 (1965)
Washizu, Y.: Single spinal motoneurons excitable from two different antidromic pathways. Jap. J. Physiol. 10, 121–131 (1960)
Waxman, S.G., Pappas, G.D.: An electron microscopic study of synaptic morphology in the oculomotor nuclei of three inframammalian species. J. comp. Neurol. 143, 41–72 (1971)
Wilson, V.J., Kato, M., Peterson, B.W., Wylie, R.M.: A single-unit analysis of the organization of Deiters' nucleus. J. Neurophysiol. 30, 603–619 (1967)
Wilson, V.J., Wylie, R.M.: A short-latency labyrinthine input to the vestibular nuclei in the pigeon. Science 168, 124–127 (1970)
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Precht, W., Richter, A., Ozawa, S. et al. Intracellular study of frog's vestibular neurons in relation to the labyrinth and spinal cord. Exp Brain Res 19, 377–393 (1974). https://doi.org/10.1007/BF00234462
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DOI: https://doi.org/10.1007/BF00234462