Summary
Membrane electrical constants have been studied in neurons of the red nucleus (RN) of the cat which were identified antidromically from the spinal cord. For each cell, the input resistance was determined from the membrane potential changes to current steps and was found to be 2.5 ± 0.9 MΩ in twenty five RN cells studied. In addition, linear summation of the membrane responses induced by two current pulses was demonstrated.
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2.
From the membrane voltage transients to current steps, the first membrane time constant, τ0, and second time contant, τ1, were determined as 5.6 ±1.0 msec and 0.6 ± 0.2 msec, respectively. The ratio of the amplitudes of two exponential functions, E1/E0, was 0.18 ± 0.05. A linear relation was found between the ratio of these amplitudes of exponential functions and that of the two time constants.
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3.
The cable parameter (electrotonic length, L) of the combined soma and dendrites of the RN neurons was estimated as 1.1 from membrane transient data using the relation developed by Rall (1969).
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4.
By using this parameter, an attempt was made to estimate the location of the two excitatory inputs on the soma-dendritic membrane of RN cells.
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Araki, T., Otani, T.: Responses of single motoneurones to direct stimulation in toad's spinal cord. J. Neurophysiol. 18, 472–485 (1955)
Barrett, J.N., Crill, W.E.: Specific membrane properties of cat motoneurones. J. Physiol. (Lond.) 239, 301–324 (1974)
Bellman, R., Astrom, K.J.: On structural identificability. Math. Biosci. 7, 329–339 (1970)
Burke, R.E.: Composite nature of the monosynaptic excitatory postsynaptic potential. J. Neurophysiol. 30, 1114–1137 (1967)
Burke, R.E., Bruggencate, G. Ten: Electrotonic characteristics of alpha motoneurones of varying size. J. Physiol. (Lond.) 212, 1–20 (1971)
Coombs, J.S., Curtis, D.R., Eccles, J.C.: The electrical constants of the motoneurone membrane. J. Physiol. (Lond.) 145, 505–528 (1959)
Coombs, J.S., Eccles, J.C., Fatt, P.: The electrical properties of the motoneurone membrane. J. Physiol. (Lond.) 130, 291–325 (1955)
Falk, G., Fatt, P.: Linear electrical properties of striated muscle fibres observed with intracellular electrodes. Proc. Roy. Soc. B 160, 69–123 (1964)
Frank, K., Fuortes, M.G.F.: Stimulation of spinal motoneurones with intracellular electrodes. J. Physiol. (Lond.) 134, 451–470 (1956)
Ito, M.: New electric device for stimulating and recording potentials for a single spinal ganglion cell. Medical Electronics. Ed. by C.N. Smith, p. 84–85. London: Liffe 1960
Ito, M., Oshima, T.: Electrical behaviour of the motoneurone membrane during intracellularly applied current steps. J. Physiol. (Lond.) 180, 607–635 (1965)
Jack, J.J.B., Redman, S.J.: The propagation of transient potentials in some linear cable structures. J. Physiol. (Lond.) 215, 283–320 (1971)
King, J.S., Martin, G.F., Conner, J.B.: A light and electron microscopic study of corticorubral projections in the opossum (Didelphis marsupialis virginiana). Brain Res. 38, 251–265 (1972)
Lux, H.D., Pollen, D.A.: Electrical constants of neurons in the motor cortex of the cat. J. Neurophysiol. 29, 207–220 (1966)
Lux, H.D., Schubert, P., Kreutzberg, G.W.: Direct matching of morphological and electrophysiological data in cat spinal motoneurones. In: Excitatory Synaptic Mechanisms. Ed. by P. Andersen, J.K.S. Jansen. Oslo: Universitetsforlaget 1970
Massion, J.: Contribution à l'étude de la régulation cérébelleuse du système extrapyramidal. Contrôle réflexe et tonique de la voie rubrospinale par le cervelet. Bruxelles: Editions Arscia S.A. Paris: Masson et Cie. 1961
Nakamura, Y., Mizuno, N.: An electron microscopic study of the interpositorubral connections of the cat and rabbit. Brain Res. 35, 283–286 (1971)
Nelson, P.G., Frank, K.: Anomalous rectification in cat spinal motoneurons and the effect of polarizing currents on the excitatory postsynaptic potential. J. Neurophysiol. 130, 1097–1113 (1967)
Nelson, P.G., Lux, H.D.: Some electrical measurements of motoneuron parameters. Biophys. J. 10, 55–73 (1970)
Nishioka, S., Nakahama, H.: Peripheral somatic activation of neurons in the cat red nucleus. J. Neurophysiol. 36, 296–307 (1973)
Rall, W.: Branching dendritic trees and motoneuron membrane resistivity. Exp. Neurol. 1, 491–527 (1959)
Rall, W.: Membrane potential transients and membrane time constant of motoneurons. Exp. Neurol. 2, 503–532 (1960)
Rall, W.: Theory of physiological properties of dendrites. Ann. N. Y. Acad. Sci. 96, 1071–1092 (1962)
Rall, W.: Theoretical significance of dendritic trees for neuronal input-output relations. In: Neural Theory and Modeling. Ed. by R.F. Reiss. Stanford University Press 1964
Rall, W.: Distinguishing theoretical synaptic potentials computed for different soma-dendritic distributions of synaptic input. J. Neurophysiol. 30, 1138–1168 (1967)
Rall, W.: Time constants and electrotonic length of membrane cylinders and neurons. Biophys. J. 9, 1483–1508 (1969)
Rall, W., Burke, R.E., Smith, T.G., Nelson, P.G., Frank, K.: Dendritic location of synapses and possible mechanisms for the monosynaptic EPSP in motoneurons. J. Neurophysiol. 30, 1169–1193 (1967)
Spencer, W.A., Kandel, E.R.: Electrophysiology of hippocampal neurons. III. Firing level and time constant. J. Neurophysiol. 24, 260–271 (1961)
Takahashi, K.: Slow and fast groups of pyramidal tract cells and their respective membrane properties. J. Neurophysiol. 28, 908–924 (1965)
Toyama, K., Tsukahara, N., Kosaka, K., Matsunami, K.: Synaptic excitation of red nucleus neurones by fibres from interpositus nucleus. Exp. Brain Res. 11, 187–198 (1970)
Tsukahara, N., Kosaka, K.: The mode of cerebral excitation of red nucleus neurons. Exp. Brain Res. 5, 102–117 (1968)
Tsukahara, N., Toyama, K., Kosaka, K.: Electrical activity of the red nucleus neurons investigated with intracellular microelectrodes. Exp. Brain Res. 4, 18–33 (1967)
Tsukahara, N., Fuller, D.R.G.: Conductance changes during pyramidally induced postsynaptic potentials in the red nucleus neurons. J. Neurophysiol. 32, 35–42 (1969)
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Dr. H. Hultborn was supported by the Japan Society for the Promotion of Science.
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Tsukahara, N., Murakami, F. & Hultborn, H. Electrical constants of neurons of the red nucleus. Exp Brain Res 23, 49–64 (1975). https://doi.org/10.1007/BF00238728
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DOI: https://doi.org/10.1007/BF00238728