Summary
Degeneration of afferent and efferent fibres supplying leg muscles was elicited by applying dry ice to the sciatic nerve in the mid femoral region. Two months after the denervation the cats showed practically normal walking activity. After 3 months the twitch tension of the reinnervated muscle (triceps surae) was almost similar to the tension developed by the non-operated contralateral control muscle.
Afferent discharges with a pattern typical for muscle spindles could rarely be found before the end of the 4th month. The muscle spindles which could be recorded before the end of the 6th month showed mainly phasic responses to ramp-and-hold stretches and only a few of them exhibited a pause during muscle contraction. None of them showed any sign of gamma innervation, as tested by several independent gamma-activating or gamma-blocking procedures.
The conduction velocities of the regenerated afferent and efferent fibres were lower than those of the normal fibres; they gradually recovered with time after the operation but did not reach normal values by the end of the 6th month. Fibre histograms revealed that the afferent fibres of groups I, II and III had regenerated, while of the efferent fibres only those corresponding to the alpha group had regenerated. Since during the period of observation the reinnervated muscles lack the support of a functioning gamma innervation, it is proposed to call them “alpha muscles”.
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References
Berry, C.M., Grundfest, H., Hinsey, J.C: The electrical activity of regenerating nerves in the cat. J. Neurophysiol. 7, 103–115 (1944)
Bessou, P., Laporte, Y., Pagès, B.: Observations sur la ré-innervation de fuseaux neuro-musculaires de Chat. C. R. Soc. Biol. (Paris) 160, 408–411 (1966)
Boyd, I.A., Davey, M.R.: The groups of origin in the nerves to skeletal muscle of the gamma 1 and gamma 2 fusimotor fibres present close to, and within, mammalian muscle spindles. In: Symposium on Muscle Receptors. (ed. D. Barker), pp. 191–198. Hong Kong: Univ. Press 1962
Brausch, U., Henatsch, H.-D., Student, C., Takano, K.: Effect of diazepam on development of stretch reflex tension. In: The Benzodiazepines. (eds. S. v. Garattini, M. Mussini and L.O. Randall), pp. 531–543. New York: Raven Press 1973
Brown, M.C., Butler, R.G.: Evidence for innervation of muscle spindle intrafusal fibres by branches of alpha motoneurones following nerve injury. J. Physiol. (Lond.) 238, 41–43 (1974)
De Santis, M., Whetsell, W.O.J.R., Francis, K.: Localization of normal and reinnervated mammalian muscle spindles for microscopy. Brain Res. 39, 240–244 (1972)
Granit, R.: Receptors and Sensory Perception. New Haven: Yale University Press 1955
Granit, R.: The Basis of Motor Control. London and New York: Academic Press 1970
Granit, R., Job, C., Kaada, B.R.: Activation of muscle spindles in pinna reflex. Acta. physiol. scand. 27, 161–168 (1952)
Homma, S.: Peripheral nerve regeneration and muscle receptor reconstruction. Electroenceph. clin. Neurophysiol. 27, 720 (1969)
Kano, M., Takano, K.: Gamma activity in the tetanus rigidity. J. physiol. soc. Japan 31, 53–54 (1969)
Matthews, P.B.C., Rushworth, G.: The selective effect of procaine on the stretch reflex and tendon jerk of soleus muscle when applied to its nerve. J. Physiol. (Lond.) 135, 246–262 (1957)
Pollock, L.J., Davis, L.: The reflex activities of a decerebrate animal. J. comp. Neurol. 50, 377–411 (1930)
Schomburg, E.D., Henatsch, H.-D.: Aktivierung lumbaler Extensor-Fusimotoneurone durch mechanische und elektrische Pinna-Reizung an decerebrierten Katzen. Pflügers Arch. 304, 152–163 (1968)
Sherrington, C.S.: On the anatomical constitution of nerves of skeletal muscles; with remarks on recurrent fibres in the ventral spinal nerve-root. J. Physiol. (Lond.) 17, 211–258 (1895)
Sherrington, C.S.: Reflexes elicitable from the pinna, vibrissae, and jaws in the cat. J. Physiol. (Lond.) 51, 404–431 (1917)
Takano, K.: Gamma activity of local tetanus in the early stage. Electroenceph. clin. Neurophysiol. 34, 803 (1973)
Takano, K., Henatsch, H.-D.: Tension-extension diagram of the tetanus intoxicated muscle of the cat. Naunyn-Schmiedebergs Arch. Pharmacol. 276, 421–436 (1973)
Takano, K., Kano, M.: Reflex activity on gamma system of rigid cat in tetanus intoxication. J. physiol. soc. Japan 30, 122 (1968)
Takano, K., Kano, M.: Gamma-bias of the muscle poisoned by tetanus toxin. NaunynSchmiedebergs Arch. Pharmacol. 276, 413–420 (1973)
Takano, K., Student, J.C: Reflex activity of the re-innervated cat muscle (I): The pattern of afferent discharges and the conduction velocity of afferent and efferent fibres. Pflügers Arch. 347, R 75 (1974)
Tower, S.S.: Atrophy and degeneration in the muscle spindle. Brain 55, 77–90 (1932)
Zelená, J., Hník, P.: Effect of innervation on the development of muscle receptors. In: The effect of use and disuse on neuromuscular functions. (eds. E. Gutmann and P. Hník), pp. 95–109. Amsterdam: Elsevier Publ. Co. 1963
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Takano, K. Absence of the gamma-spindle loop in the reinnervated hind leg muscles of the cat: “Alpha-Muscle”. Exp Brain Res 26, 343–354 (1976). https://doi.org/10.1007/BF00234218
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DOI: https://doi.org/10.1007/BF00234218