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Afferent fiber connections from lower brain stem to hypothalamus studied by the horseradish peroxidase method with special reference to noradrenaline innervation

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Summary

Attempts were made to determine the afferent projections to the anterior hypothalamus including the preoptic area from the lower brain stem by means of the horseradish peroxidase method combined with monoamine oxidase staining to identify noradrenaline (NA) neurons. In addition to this technique, a histofluorescence analysis was performed. NA fibers in the medial part of the anterior hypothalamus were mainly supplied by A1 and A2 NA neuron groups, while the lateral part and periventricular zone received NA terminals from both pontine and medulla oblongata NA neuron groups. Furthermore, the present study indicated that there were direct projections to the anterior hypothalamus from non-noradrenergic neurons in the lower brain stem: nuclei raphe dorsalis, centralis superior, cells in the mesencephalic and pontine central gray matter, nuclei parabrachialis lateralis and medialis, cells around fasciculus longitudinalis medialis.

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Abbreviations

CA:

Commissura anterior

CO:

Chiasma opticum

DP:

Decussatio pyramidum

DPCS:

Decussatio pedunculorum cerebellarium superiorum

F:

Columna fornicis

FLM:

Fasciculus longitudinalis medialis

FMT:

Fasciculus mamillothalamicus

GCM:

Griseum centrale mesencephali

GCP:

Griseum centrale pontis

LL:

Lemniscus lateralis

LM:

Lemniscus medialis

PCM:

Pedunculus cerebellaris medius

PCS:

Pedunculus cerebellaris superior

TO:

Tractus opticus

TS:

Tractus solitarius

TVme:

Tractus mesencephalicus nervi trigemini

V:

Ventriculus tertius

VTS:

Tractus spinalis nervi trigemini

am:

nucleus ambiguus

B:

Barrington nucleus

com:

nucleus commissuralis

cp:

nucleus caudatus putamen

cs:

nucleus centralis superior

ct:

nucleus corporis trapezoidei

cu:

nucleus cuneatus

dX:

nucleus dorsalis nervi vagi

Gd:

nucleus tegmentalis dorsalis (von Gudden)

gr:

nucleus gracilis

Gv:

nucleus tegmentalis ventralis (von Gudden)

ha:

nucleus hypothalamicus anterior

hl:

nucleus hypothalamicus lateralis

hpe:

nucleus periventricularis (hypothalami)

hvm:

nucleus ventromedialis hypothalami

lc:

nucleus locus coeruleus

oi:

nucleus olivaris inferior

p:

nucleus pontis

pa:

nucleus paraventricularis

pbl:

nucleus parabrachialis lateralis

pbm:

nucleus parabrachialis medialis

ph:

nucleus praepositus hypoglossi

pol:

nucleus preopticus lateralis

pom:

nucleus preopticus medialis

pop:

nucleus preopticus periventricularis

rd:

nucleus raphe dorsalis

re:

nucleus reuniens

rl:

nucleus reticularis lateralis

rm:

nucleus raphe magnus

ro:

nucleus raphe obscrus

sc:

nucleus suprachiasmaticus

so:

nucleus supraopticus

st:

nucleus interstitialis striae terminalis

td:

nucleus tractus diagonalis (Broca)

ts:

nucleus tractus solitarii

Vme:

nucleus mesencephalicus nervi trigemini

Vmo:

nucleus motorius nervi trigemini

Vts:

nucleus tractus spinalis nervi trigemini

XII:

nucleus nervi hypoglossi

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Authors and Affiliations

  1. Department of Neuroanatomy, Institute of Higher Nervous Activity, Osaka Medical School, 33 Joan-cho, Kitaku, Osaka, Japan

    T. Sakumoto, M. Tohyama, K. Satoh, Y. Kimoto & N. Shimizu

  2. Department of Obstetrics and Gynecology, Osaka University Medical School, Osaka Medical School, 33, Joan-cho, Kitaku, Osaka, Japan

    T. Kinugasa, O. Tanizawa & K. Kurachi

Authors
  1. T. Sakumoto
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  2. M. Tohyama
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  3. K. Satoh
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  4. Y. Kimoto
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  5. T. Kinugasa
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  6. O. Tanizawa
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  7. K. Kurachi
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  8. N. Shimizu
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Sakumoto, T., Tohyama, M., Satoh, K. et al. Afferent fiber connections from lower brain stem to hypothalamus studied by the horseradish peroxidase method with special reference to noradrenaline innervation. Exp Brain Res 31, 81–94 (1978). https://doi.org/10.1007/BF00235806

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