Summary
Distribution of substance P (SP)-positive fibers in the medial preoptic area (POM) of the rat and their origins were examined using indirect immunofluorescence. A very high density of SP-positive fibers was seen in the POM throughout its entire rostro-caudal extent. However, the distribution of these fibers was not even; the highest density was detected in the medial part of the POM, with less dense but still numerous fibers in the lateral part. On the other hand, in this area a small number of SP-positive cells could be found; a few cells were scattered in the rostral part and, in the caudal part, several cells could be seen in the ventral part of the POM.
The origins of SP-positive fibers in the POM were experimentally examined. Since the destruction of the ventro-lateral part of the anterior hypothalamus (vlAH), where numerous SP-positive cells were seen, resulted in a marked decrease of SP-positive fibers in the POM on the operated side, the majority of these fibers may originate from SP-positive cells in the vlAH.
The fine structure of SP-positive terminals in the POM were investigated by electron-microscopic immunohistochemical techniques. Immunoreactive terminals contained a few large granular vesicles together with numerous small vesicles, and they made synaptic contacts mainly with dendrites which were devoid of immunoreactive materials. Two different synaptic contacts could be distinguished: one asymmetrical (Gray's type I) and the other symmetrical (Gray's type II), with the latter being predominant.
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References
Bean NJ, Nunez AA, Conner R (1981) Effects of medial preoptic lesions on male mouse ultrasonic vocalizations and copulatory behavior. Brain Res Bull 6: 109–112
Clemens JA, Smalstig EB, Sawyer BD (1976) Studies on the role of the preoptic area in the control of reproductive function in the rat. Endocrinology 99: 728–735
Coons AH (1958) Fluorescent antibody methods. In: Danielli JF (ed) General cytochemical methods. Academic Press, New York, pp 399–422
Cox B, Lee TF (1977) Do central dopamine receptors have a physiological role in thermoregulation? Br J Pharmacol 61: 83–86
Cuello AC, Kanazawa I (1978) The distribution of substance P-immunoreactive fibers in the rat central nervous system. J Comp Neurol 178: 129–156
Cytawa J, Teitelbaum P (1967) Spreading depression and recovery of subcortical functions. Acta Biol Exp (Warsaw) 27: 345–353
Day TA, Willoughby JO, Geffen LB (1979) Thermoregulatory effects of preoptic area injections of noradrenaline in restrained and unrestrained rats. Brain Res 174: 175–179
Gray EG (1961) Ultrastructure of synapses of the cerebral cortex and of certain specializations of the neuroglial membranes. In: Boyd ID, Johnson FR, Lever JD (eds) Electron microscopy in anatomy. Arnold, London, pp 54–73
Hökfelt T, Elde RP, Johansson O, Kellerth J-O, Ljungdahl Å, Nilsson G, Pernow B, Terenius L (1977a) Substance P and enkephalin: Distribution in the nervous system as revealed with immunohistochemistry. In: Radouco-Thomas C (ed) College of international neuropsychopharmacology. Raven Press, New York, pp 477–487
Hökfelt T, Johansson O, Kellerth J-O, Ljungdahl Å, Nilsson G, Nygards A, Pernow B (1977b) Immunohistochemical distribution of substance P. In: Von Euler US, Pernow B (eds) Substance P. Nobel Symposium 37. Raven Press, New York, pp 117–145
Hökfelt T, Kellerth J-O, Ljungdahl Å, Nilsson G, Nygards A, Pernow B (1977c) Immunohistochemical localization of substance P in the central and peripheral nervous system. In: Barchas J, Costa E, Usdin E (eds) Neuroregulators and hypotheses of psychiatric disorders. Oxford University Press, Oxford, pp 299–310
Inagaki S, Sakanaka M, Shiosaka S, Senba E, Takatsuki K, Takagi H, Kawai Y, Minagawa H, Tohyama M (1982) Ontogeny of substance P-containing neuron system of the rat: Immunohistochemical analysis. I. Forebrain and upper brain stem. Neuroscience 7: 251–277
Jacobowitz DM, Palcovits M (1974) Topographic atlas of catecholamine and acetylcholinesterase-containing neurons in the rat brain. I. Forebrain (Telencephalon, diencephalon). J Comp Neurol 157: 13–28
König JFR, Klippel RA (1963) The rat brain. A stereotaxic atlas of the forebrain and lower parts of the brain stem. Williams and Wilkins, Baltimore
Leedy MG, Vela EA, Popolow HB, Gerall AA (1980) Effect of prepuberal medial preoptic area lesions on male rat sexual behavior. Physiol Behav 24: 341–346
Ljungdahl Å, Hökfelt T, Nilsson G (1978a) Distribution of substance P-like immunoreactivity in the central nervous system of the rat. I. Cell bodies and nerve terminals. Neuroscience 3: 861–943
Ljungdahl Å, Hökfelt T, Nlisson G, Goldstein M (1978b) Distribution of substance P-like immunoreactivity in the central nervous system of the rat. II. Light microscopic localization in relation to catecholamine-containing neurons. Neuroscience 3: 945–976
Malsbury CW (1971) Facilitation of male rat copulatory behavior by electrical stimulation of the medial preoptic area. Physiol Behav 7: 785–797
Mesulam M-M (1978) Tetramethylbenzidine for horseradish peroxidase neurohistochemistry: A non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117
Noonan M, Kristal MB (1979) Effects of medial preoptic lesions on placentophagia and on the onset of maternal behavior in the rat. Physiol Behav 22: 1197–1202
Numan M, Rosenblatt JS, Komisaruk BR (1977) Medial preoptic area and onset of maternal behavior in the rat. J Comp Physiol Psychol 91: 146–164
Paxinos G, Emson PC, Cuello AC (1978) Substance P projections to the entopeduncular nucleus, the medial preoptic area and lateral septum. Neurosci Lett 7: 133–136
Sakanaka M, Shiosaka S, Takatsuki K, Inagaki S, Takagi H, Senba E, Kawai Y, Matsuzaki T, Tohyama M (1981) Experimental immunohistochemical studies on the amygdalofugal peptidergic (substance P and somatostatin) fibers in the stria terminalis of the rat. Brain Res 221: 231–242
Satinoff E, Valentino D, Teitelbaum P (1976) Thermoregulatory cold-defense deficits in rats with preoptic/anterior hypothalamic lesions. Brain Res Bull 1: 553–565
Sherwood C, Massopust LC, McCrum WR, Buchanan AR (1954) The effect of hypothalamic lesions upon body temperature maintenance in the albino rat while in cold environments. J Neuropath Exp Neurol 13: 191–208
Somogyi P, Takagi H (1982) A note on the use of picric acidparaformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic irnmunocytochemistry. Neuro science 7: 1779–1783
Sternberger LA, Hardy PH, Curculis JJ, Meyer HG (1970) The unlabeled antibody-enzyme method of immunocytochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18: 315–333
Ungerstedt U (1971) Stereotaxic mapping of the monoamine pathways in the rat brain. Acta Physiol Scand [Suppl] 367: 1–48
Versteeg DHG, Van der Gugten J, De Jong W, Palkovits M (1976) Regional concentrations of noradrenaline and dopamine in rat brain. Brain Res 133: 563–574
Zamboni L, De Martino C (1967) Buffered picric-acid formaldehyde: A new rapid fixative for electron microscopy. J Cell Biol 35: 148A
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Supported in part by a grant-in-aid from the Ministry of Education, Science and Culture of Japan (57370002)
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Takatsuki, K., Sakanaka, M., Takagi, H. et al. Experimental immunohistochemical studies on the distribution and origins of substance P in the medial preoptic area of the rat. Exp Brain Res 53, 183–192 (1983). https://doi.org/10.1007/BF00239411
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DOI: https://doi.org/10.1007/BF00239411