Summary
The origins of calcitonin gene-related peptide immunoreactive (CGRPI) fibers in the cochlea were examined in rats. Parasagittal transection of the brain just medial to the principal sensory trigeminal nucleus resulted in the ipsilateral disappearance of CGRPI fibers in the cochlea, indicating that the origins of these fibers lie in the central nervous system. Next, we used a highly sensitive method combining retrograde tracing and immunohistochemistry to identify the origins of the CGRPI fibers in the cochlea. After injection of biotin-wheat germ agglutinin (b-WGA) into the cochlea, CGRPI neurons in the ipsilateral lateral superior olivary nucleus also contained b-WGA granules. These findings indicated tht CGRPI efferent fibers are major components of the olivocochlear bundle.
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Altschuler RA, Parakkal MH, Fex J (1983) Localization of enkephalin-like immunoreactivity in acetylcholinesterasepositive cells in guinea pig lateral superior olivary complex that project to the cochlea. Neuroscience 9: 621–630
Altschuler RA, Parakkal MH, Rubio JA, Hoffman DW, Fex J (1984a) Enkephalin-like immunoreactivity in the guinea pig organ of Corti: ultrastractural and lesion studies. Hearing Res 16: 17–31
Altschuler RA, Fex J, Parakkal MH, Eckenstein F (1984b) Colocalization of enkephalin-like and choline acetyltransferase-like immunoreactivities in olivocochlear neurons of the guinea pig. J Histochem Cytochem 32: 839–843
Altschuler RA, Kachar B, Rubio JA, Parakkal MH, Fex J (1985) Immunocytochemical localization of choline acetyltransferase-like immunoreactivity in the guinea pig cochlea. Brain Res 338: 1–11
Amara SG, Jonas V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature 298: 240–244
Coons AH (1958) Fluorescent antibody method. In: Dabielli JF (ed) General cytochemical methods. Academic Press, New York, pp 399–422
Fex J, Altschuler RA (1981) Enkephalin-like immunoreactivity of olivocochlear nerve fibers in cochlea of guinea pig and cat. Proc Natl Acad Sci USA 78: 1255–1259
Kawai Y, Takami K, Shiosaka S, Emson PC, Hillyard CJ, Girgis S, MacIntyre I, Tohyama M (1985) Topographic localization of calcitonin gene-related peptide in the rat brain: an immunohistochemical analysis. Neuroscience 15: 747–763
Kitajiri M, Yamashita T, Tohyama Y, Kumazawa T, Takeda N, Kawasaki Y, Matsunaga T, Girgis S, Hillyard CJ, Maclntyre I, Emson PC, Shiosaka S, Tohyama M (1985) Localization of calcitonin gene-related peptide in the organ of Corti of the rat: an immunohistochemical study. Brain Res 358: 394–397
Mason RT, Peterfreund RA, Sawchenko PE, Corrigan AZ, Rivier JE, Vale WW (1984) Release of the predicted calcitonin generelated peptide from cultured rat trigeminal ganglion cells. Nature 308: 653–655
Morris HR, Panico E, Etienne T, Tippimns J, Girgis SI, MacIntyre I (1984) Isolation and characterization of human calcitonin gene-related peptide. Nature 308: 746–748
Rosenfeld MG, Mermord J-J, Amara SG, Swanson LW, Sawchenko PE, Rivier J, Vale WW, Evans RM (1983) Production of a novel neuropeptide encoded by the calcitonin gene via tissue-specific RNA processing. Nature 304: 129–135
Rasmussen GL (1946) The olivary peduncle and other fiber projections of the superior olivary complex. J Comp Neurol 84: 141–219
Rasmussen GL (1953) Further observations of the efferent cochlear bundle. J Comp Neurol 99: 61–74
Rasmussen GL (1960) Efferent fibers of the cochlear nerve and cochlear nucleus. In: Rasmussen GL, Windle WF (eds) Neural mechanisms of the auditory and vestibular system. Thomas, Springfield, pp 105–115
Shiosaka S, Shibasaki T, Tohyama M (1984) Bilateral α-melanocyte stimulating hormonergic fiber system from the zona incerta to cerebral cortex: combined retrograde axonal transport and immunohistochemical study. Brain Res 309: 350–353
Shiosaka S, Shimada S, Tohyama M (1986) Sensitive doublelabeling technique of retrograde biotinized tracer (biotin-WGA) and immunocytichemistry: light and electron microscopic analysis. J Neurosci Meth (in press)
Warr WB (1975) Olivocochlear and vestibular efferent neurons of the feline brain stem: their localization, morphology and number determined by retrograde axonal transport and acetylcholinesterase histochemistry. J Comp Neurol 161: 159–182
Warr WB, Guinan JJ (1979) Efferent innervation of the organ of Corti: two separate systems. Brain Res 173: 152–155
Zamboni L, De Martino C (1967) Buffered picric acid formaldehyde: a new rapid fixative for electron microscopy. J Cell Biol 35: 148A
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Takeda, N., Kitajiri, M., Girgis, S. et al. The presence of a calcitonin gene-related peptide in the olivocochlear bundle in rat. Exp Brain Res 61, 575–578 (1986). https://doi.org/10.1007/BF00237583
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DOI: https://doi.org/10.1007/BF00237583