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Estrogen-induced alterations in synaptic morphology in the midbrain central gray

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Summary

Axons of ventromedial hypothalamic (VMH) neurons have been previously shown to terminate in the midbrain central gray (MCG) (Chung et al. 1984, 1986). Since VMH synapses in this region may be involved in the mediation of estrogen-induced lordosis behavior, we examined the effect of estrogen on the morphology of synapses in the MCG. Ovariectomized adult female rats were given daily subcutaneous injections of estradiol benzoate (10 μg) or the vehicle control and after 20 days of injection, only the estrogen-treated rats showed the lordosis response. A quantitative analysis of MCG tissue from these animals demonstrates morphological changes in various synaptic parameters with estrogen treatment including: 1) an increase in the mean number of dense-cored vesicles and an increase in the number of terminals containing densecored vesicles, 2) an increase in the length of postsynaptic densities (PSDs), 3) an increase in the number of PSDs showing perforations, 4) an increase in the number of synapses, and 5) an increase in the number of synapses with positive synaptic curvature. No alterations in the number of subjunctional bodies were observed. The dense-cored vesicles may contain an estrogen-induced trophic factor which may function in maintaining the integrity of postsynaptic processes and cells in the MCG with which VMN endings contact (Chung et al. submitted) and/or which induces morphological changes in postsynaptic structures which facilitate the effects of estrogen on lordosis behavior.

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Authors and Affiliations

  1. Department of Anatomy, Universtiy of Illinois at Chicago, 808 S. Wood St., 60612, Chicago, IL, USA

    S. K. Chung & R. S. Cohen

  2. Department of Neurobiology and Behavior, The Rockefeller University, 1230 York Avenue, 10021, New York, NY, USA

    D. W. Pfaff

Authors
  1. S. K. Chung
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  2. D. W. Pfaff
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  3. R. S. Cohen
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Chung, S.K., Pfaff, D.W. & Cohen, R.S. Estrogen-induced alterations in synaptic morphology in the midbrain central gray. Exp Brain Res 69, 522–530 (1988). https://doi.org/10.1007/BF00247306

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  • DOI: https://doi.org/10.1007/BF00247306

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