Summary
Light and electron microscopic analysis of calcitonin gene-related peptide (CGRP)-like immunoreactive (LI) terminals in the bed nucleus of the stria terminalis (BST) and the central nucleus of the amygdala (Ce) was carried out using the peroxidase-antiperoxidase method. CGRP-LI fibers were densely distributed in the dorsal subdivision of the lateral BST (BSTL) and the lateral and lateral capsular subdivisions of the Ce, where the CGRP-LI terminals formed symmetrical and asymmetrical axo-dendritic, and symmetrical axosomatic synapses. One of the most characteristic features of the CGRP-LI terminals was the presence of large, long boutons, each of which surrounded a cell soma and made many synaptic contacts. These findings suggest that CGRP exerts a significant influence on neurons in the BSTL and Ce.
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References
Amara SG, Jonas V, Rosenfeld MG, Ong ES, Evans RM (1982) Alternative RNA processing in calcitonin gene expression generates mRNA encoding different polypeptide products. Nature (London) 298:240–244
Bodian D (1972) Synaptic diversity and characterization by electron microscopy. In: Pappas GD, Purpura DP (eds) Structure and function of synapses. Raven Press, New York, pp 45–65
Bolam JP, Somogyi P, Totterdell S, Smith AD (1981) A second type of striatonigral neuron: a comparison between retrogradely labeled and Golgi-stained neurons at the light and electron microscopic levels. Neuroscience 6:2141–2157
Cohen MI (1971) Switching of respiratory phases and evoke phrenic responses produced by rostral pontine electrical stimulation. J Physiol (London) 217:133–158
Gray TS, Cassell MD, Nilaver G, Zimmerman EA, Williams TH (1984) The distribution and ultrastructure of VIP-immunoreactivity in the central nucleus of the rat amygdala. Neuroscience 11:399–408
Harper RM, Frysinger RC, Trelease RB, Marks LDJ (1984) State dependent alteration of respiratory cycle timing by stimulation of the central nucleus of the amygdala. Brain Res 306:1–8
Henke PG (1980) The centromedial amygdala and gastric pathology in rats. Physiol Behav 25:107–112
Innes DL, Tansy MF (1980) Gastric mucosal ulceration associated with electrochemical stimulation of limbic brain. Brain Res Bull 5:33–36
Kawai Y, Takami K, Shiosaka S, Emson PC, Hillyard CJ, Girgis S, MacIntyre I, Tohyama M (1985) Topographic localization of calcitonin gene-related peptide in the rat brain: an immunohistochemical analysis. Neuroscience 15:747–763
Mason RT, Pelerfeund RA, Sawchenko PE, Corrigan AZ, Rivier JE, Vale WW (1984) Release of the predicted calcitonin gene-related peptide from cultured rat trigeminal ganglion cells. Nature (London) 308:653–655
Morris HR, Panico M, Etienne T, Tippins J, Girgis SI, MacIntyre I (1984) Isolation and characterization of human calcitonin gene-related peptide. Nature (London) 308:746–748
Norgren R (1976) Taste pathway of hypothalamus and amygdala. J Comp Neurol 166:17–30
Nguyen KQ, Sills MA, Jacobowitz DM (1986) Cardiovascular effects produced by microinjection of calcitonin gene-related peptide into the rat central amygdaloid nucleus. Peptides 7:337–339
Rosenfeld MG, Mermod J-J, Amara SG, Swanson LW, Sawchenko PE, Rivier J, Vale WW, Evans RM (1983) Production of novel neuropeptide encoded by the calcitonin gene via tissuespecific RNA processing. Nature (London) 304:129–135
Shimada S, Shiosaka S, Emson PC, Hillyard CJ, Girgis S, MacIntyre I, Tohyama M (1985) Calcitonin gene-related peptidergic projection from the parabrachial area to the forebrain and diencephalon in the rat: an immunohistochemical analysis. Neuroscience 16:607–616
Skofitsch G, Jacobowitz DM (1985) Calcitonin gene-related peptide: detailed immunohistochemical distribution in the central nervous system. Peptides 6:721–745
Somogyi P, Takagi H (1982) A note on the use of picric acidparaformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic immunohistochemistry. Neuroscience 7:1779–1783
Yamamoto T (1983) Neural mechanisms of the taste function. Front Oral Physiol 4:102–103
Yamano M, Hillyard CJ, Girgis S, MacIntyre I, Emson PC, Tohyama M (1988a) Presence of a substance P-like immunoreactive neurone system from the parabrachial area to the central amygdaloid nucleus of the rat with reference to coexistence with calcitonin gene-related peptide. Brain Res 451:179–188
Yamano M, Hillyard CJ, Girgis S, Emson PC, MacIntyre I, Tohyama M (1988b) Projection of neurotensin-like immunoreactive neurons from the lateral parabrachial area to the central amygdaloid nucleus of the rat with reference to the coexistence with calcitonin gene-related peptide. Exp Brain Res 71:603–610
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Shimada, S., Inagaki, S., Kubota, Y. et al. Light and electron microscopic studies of calcitonin gene-related peptide-like immunoreactive terminals in the central nucleus of the amygdala and the bed nucleus of the stria terminalis of the rat. Exp Brain Res 77, 217–220 (1989). https://doi.org/10.1007/BF00250584
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DOI: https://doi.org/10.1007/BF00250584