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Quantitative distribution of rat brain monoamine oxidase A by [14C]clorgyline autoradiography

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Abstract

The distribution of functionally active monoamine oxidase type A (MAO-A) was investigated by in vivo quantitative autoradiography using [14C]clorgyline in normal, conscious rat brain. [14C]clorgyline was synthesized by the methylation reaction of N-desmethylclorgyline using [14C]methyliodide. Sixty minutes after [14C]clorgyline administration (1.58 MBq/animal i.v.), the brains were removed and prepared for autoradiography by washing the brain sections with 5% trichloroacetic acid solution to remove the nonbinding free tracer. The amount of MAO-A was calculated from the regional acid-insoluble tissue radioactivity and the specific activity of the tracer. The highest amount of MAO-A (5.84 nmol/g tissue) was found in the locus coeruleus. The interpeduncular nucleus, habenular nucleus, fasciculus retroflexus, and solitary tract nucleus possessed over 1.6 nmol/g tissue of MAO-A. Among 23 regions of interest, the lowest amount of MAO-A (0.37 nmol/g tissue) was found in the globus pallidus. The findings of this study suggest that the pattern of MAO-A parallels both in neuroanatomical distribution and in density that of norepinephrine and serotonin innervation. The MAO-A concentration was, however, relatively low in the dopamine-related areas. This corresponded to the previous results obtained by histochemical analysis. In addition, among the white matter structures, a high amount of MAO-A was found specifically in the fasciculus retroflexus.

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Authors and Affiliations

  1. Department of Neurology, Research Institute for Brain and Blood Vessels-Akita, 010, Akita, Japan

    Yasushi Kondoh, Weimin Yin & Ken Nagata

  2. Department of Radiology and Nuclear Medicine, Research Institute for Brain and Blood Vessels-Akita, 010, Akita, Japan

    Matsutaro Murakami

  3. Department of Internal Medicine, Research Institute for Brain and Blood Vessels-Akita, 010, Akita, Japan

    Shigenori Mizusawa & Hiroyuki Nakamichi

Authors
  1. Yasushi Kondoh
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  2. Matsutaro Murakami
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  3. Weimin Yin
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  4. Shigenori Mizusawa
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  5. Hiroyuki Nakamichi
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  6. Ken Nagata
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Kondoh, Y., Murakami, M., Yin, W. et al. Quantitative distribution of rat brain monoamine oxidase A by [14C]clorgyline autoradiography. Exp Brain Res 99, 375–382 (1994). https://doi.org/10.1007/BF00228974

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  • DOI: https://doi.org/10.1007/BF00228974

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