Skip to main content
Log in

Electrophoretic patterns of serum proteins in the blood of four species of puff-fish, genus Fugu, and their F1 hybrids

  • Published:
Marine Biology Aims and scope Submit manuscript

Abstract

In a series of hematological investigations to analyze species characteristics and ecological factors controlling the distribution of marine fish, comparisons of the electrophoretic patterns of the serum proteins in the blood of four species of puff-fish (Fugu rubripes, F. xanthopterus, F. poecilonotus and F. pardalis) and two artificial F1 hybrids (male F. rubripesxfemale F. xanthopterus; female F. poecilonotusxmale F. pardalis) were made by using the cellulose acetate membrane technique. The number of components, as well as the position and ratio of each component, differed from species to species and with sex. The serum protein pattern of the F1 hybrid was much the same as that derived from the arithmetic mean of the parental patterns. The opening made in a portion of the abdomen to sample the blood directly from the auricle could be sutured successfully by biotissue glue “Biobond”.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature cited

  • Fujita, S.: Artificial interspecific and intergeneric hybridizations among the Tetradontid puffers (Preliminary report). Michulin Biology 3 5–11 (1967).

    Google Scholar 

  • Sulya, L. L., B. E. Box, and G. Gunter: Plasma proteins in the blood of fishes from the Gulf of Mexico. Am. J. Physiol. 200, 152–154 (1961).

    Google Scholar 

  • Tsuyuki, H., and E. Roberts: Species differences of some members of Salmonidae based on their muscle myogen patterns. J. Fish. Res. Bd Can. 20, 101–104 (1963).

    Google Scholar 

  • : Zone electrophoretic comparison of muscle myogens and blood proteins of artificial hybrids of Salmonidae with their parental species. J. Fish. Res. Bd Can. 22, 767–773 (1965).

    Google Scholar 

  • : Inter-species relationships within the genus Oncorhynchus based on biochemical systematics. J. Fish. Res. Bd Can. 23, 101–107 (1966).

    Google Scholar 

  • and L. W. Clarke: Comparative electropherograms of Coregonus clupeaformis, Salvelinus namaycush, S. alpinus, S. malma, and S. fontinalis from the Family Salmonidae. J. Fish. Res. Bd Can. 23, 1599–1606 (1966).

    Google Scholar 

  • Uthe, J. F., E. Roberts, L. W. Clarke, and H. Tsuyuki: Comparative electropherograms of representatives of the families Petromyzontidae, Esocidae, Centrachidae, and Percidae. J. Fish. Res. Bd Can. 23, 1663–1671 (1966).

    Google Scholar 

  • Yamanaka, H., K. Yamaguchi, K. Hashimoto, and F. Matsuura: Starch-gel electrophoresis of fish hemoglobins-III Salmonoid fishes. Bull. Jap. Soc. scient. Fish. 33, 195–203 (1967).

    Google Scholar 

  • Yamashita, H.: Haematological study of a species of rock-fish, Sebastiscus marmoratus (C. et V.)-I. The effect of chlorinity on the moisture content and specific gravity of blood, serum protein and erythrocyte counts. Bull. Jap. Soc. scient. Fish. 33, 81–90 (1967a).

    Google Scholar 

  • : Haematological study of a species of rockfish, Sebastiscus marmoratus (C. et V.)-II. Changes of the moisture content of blood, specific gravity, serum protein, haematocrit value and urea nitrogen level of serum in the specimens having ulcers. Bull. Jap. Soc. scient. Fish 33, 995–1001 (1967b).

    Google Scholar 

  • Yamashita, H.: Haematological study of a species of rockfish, Sebastiscus marmoratus (C. et V.)-III. Changes of the serum protein fractions during storage (in preparation).

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by M. Anraku, Nagasaki

Contribution No. 220 from the Seikai Regional Fisheries Research Laboratory, Nagasaki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamashita, H. Electrophoretic patterns of serum proteins in the blood of four species of puff-fish, genus Fugu, and their F1 hybrids. Marine Biol. 1, 277–281 (1968). https://doi.org/10.1007/BF00360776

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00360776

Keywords

Navigation