Abstract
The development of intestinal lipid absorption capacities was studied in larvae of the sea bassDicentrarchus labrax from the time of mouth opening to 25 d after hatching, in individuals fed with brine shrimp,Artemia sp. (Brazil commercial strain) or artificial diets. At the time of mouth opening, before the first feed, enterocytes synthesized lipoprotein particles from endogenous lipids. Starting with the first feed, enterocytes absorbed food lipids regardless of the diet, but since these cells have a low capacity for lipoprotein synthesis, they accumulated lipids in the form of “free” lipid droplets. In larvae fed withArtemia sp., the rapid development of enterocytes during growth was combined with increasingly effective lipoprotein synthesis (becoming even greater than that observed in the adult), starting on Days 18 to 19. Although lipoprotein synthesis and transport were observed in larvae given artificial feed, they showed abnormalities in their intestinal mucosa. Lipid droplets formed in association with the membrane structures of specimens given egg yolk during the first days of feeding, and enterocytes degenerated. In 18 to 19 d-old larvae given artificial feed, intestinal folds disappeared and the constituent cells showed limited differentiation. In the last-mentioned individuals, the abnormalities appeared to be related to malnutrition resulting from inadequacy of the food offered, which led to total mortality of larvae, beginning on Day 20.
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Alliot, E., Pastoureaud, A., Trellu, J. (1977). Evolution des activités enzymatiques dans le tractus digestif au cours de la vie larvaire du bar,Dicentrarchus labrax. Variations des protéinogrammes et des zymogrammes. Publs Cent. natn. Exploit. Océans (CNEXO) (Sér. Act. Colloques) 4: 85–91
Beccaria, C. (1989). Le pancréas exocrine et endocrine du loup,Dicentrarchus labrax L.: organogenèse et variations morphofonctionnelles liées aux conditions alimentaires. Thèse. Université Sciences et Techniques du Languedoc, Montpellier, France
Berendsen, P. B., Blanchette-Mackie, E. J. (1979). Milk lipid absorption and chylomicron production in the suckling rat. Anat. Rec. 195: 397–314
Bergot, P., Fléchon, J. E. (1970a). Forme et voie d'absorption intestinale des acides gras chez la truite arc-en-ciel (Salmo gairdneri Rich.) I. Lipides en particules. Annls Biol. anim. Biochim. Biophys. 10: 459–472
Bergot, P., Fléchon, J. E. (1970b). Forme et voie d'absorption intestinale des acides gras à chaine longue chez la truite arc-enciel (Salmo gairdneri Rich.). II. Lipides “étalés”. Annls. Biol. anim. Biochim. Biophys. 10: 473–480
Connes, R., Benhalima, K. (1983). Ultrastructure de l'intestin du loupDicentrarchus labrax L. au cours du développement larvaire. Bull. Soc. zool. Fr. 109: 19–33
Deplano, M. (1989). Etude morpho-fonctionnelle de la muqueuse intestinale du loup,Dicentrarchus labrax L. Mise en évidence de pathologies nutritionnelles chez les sujets immatures et chez les larves. Thèse. Université Sciences et Techniques du Languedoc, Montpellier, France
Deplano, M., Connes, R., Diaz, J. P., Paris, J. (1989). Intestinal steatosis in the farm-reared sea bass,Dicentrarchus labrax. Dis. aquat. Organisms 6: 121–130
Dyer, F. R. (1968). Morphological features of brown adipose cell maturation in vivo and in vitro. Am. J. Anat 123: 255–282
Govoni, J. J., Boehlert, G. W., Watanabe, Y. (1986). The physiology of digestion in fish larvae. Envir. Biol. Fish. 16: 59–77
Iwai, T. (1968a). The comparative study of the digestive tract of teleost larvae. V. Fat absorption in the gut epithelium of goldfish larvae. Bull. Jap. Soc. scient. Fish. 34: 973–978
Iwai, T. (1968b). Fine structure and absorption patterns of intestinal epithelial cells in rainbow trout alevins. Z. Zellforsch. 91: 366–379
Loewe, H., Eckmann, R. (1988). The ontogeny of the alimentary tract of coregonid larvae: normal development. J. Fish Biol. 33: 841–850
Malagelada, J. R., Linscheer, W. G., Fishman, W. H. (1977). The effect of fatty acid perfusion on intestinal alkaline phosphatase. II. Studies on the rat. Am. J. dig. Dis. 22: 516–523
Ono, K., Sohma, M., Satoh, Y. (1984). Ultrastructural localization of acid phosphatase activity in the jejunal absorptive cells of fasted rats. Acta histochem. 74: 133–137
Pearse, A. G. E. (1968). Histochemistry, theoretical and applied. Churchill Livingstone, London and New York
Rombout, J. H. W. M., Stroband, H. W. J., Taverne-Thiele, J. J. (1984). Proliferation and differentiation of intestinal cells during development ofBarbus conchonius (Teleostei, Cyprinidae). Cell. Tissue Res. 236: 207–216
Segner, H., Rosch, R., Schmidt, H., von Poeppinghausen, K. J. (1989). Digestive enzymes in larvalCoregonus lavaretus L. J. Fish Biol. 35: 249–263
Sire, M. F., Vernier, J. M. (1979). Formation de VLDL intestinales endogènes. Etude ultrastructurale sur un nouveau modèle, l'embryon et l'adulte, à jeun, de truite. Biol. cell. 35: 271–280
Sire, M. F., Vernier, J. M. (1981). Etude ultrastructural de la synthèse de chylomicrons au cours de l'absorption intestinale des lipides chez la truite. Influence de la nature des acides gras ingérés. Biol. cell. 40: 47–62
Stroband, H. W. J., Dabrowski, K. R. (1979). Morphological and physiological aspects of the digestive system and feeding in fresh-water fish larvae. In: Fontaine, M. (ed.) Nutrition des poissons. Centre National de la Recherche Scientifique, Paris, p. 355–376 (Act. Colloque CNERNA)
Stroband, H. W. J., Meer, H. V. D., Timmermans, L. P. M. (1979). Regional functional differentiation in the gut of the grass carp,Ctenopharyngodon idella (Val.). Histochemistry 64: 235–249
Tanaka, M. (1972). Studies on the structure and function of the digestive system in teleost larvae. IV. Changes in the epithelium related to fat absorption in the anteromedian part of the intestine after feeding. Jap. J. Ichthyol. 19: 15–25
Vernier, J. M., Sire, M. F. (1977). Lipoprotéines de très basse densité et glycogène dans le syncytium vitellin, l'épithélium intestinal et le foie, aux stades précoces du développement embryonnaire chez la truite arc-en-ciel. Biol. cell. 29: 45–54
Vu, T. T. (1976). Etude du développement du tube digestif des larves de bar,Dicentrarchus labrax (L.). Archs Zool. exp. gén 117: 493–509
Vu, T. T. (1980). Etude histologique de l'épithélium du tube digestif du barDicentrarchus labrax (L.) au cours du développement post embryonnaire. Archs Zool. exp. gén. 121: 131–206
Vu, T. T. (1983). Etude histoenzymologique des activités protéasiques dans le tube digestif des larves et des adultes de bar,Dicentrarchus labrax (L.). Aquaculture, Amsterdam 32: 57–69
Watanabe, Y. (1985). Histological changes in the liver and intestine of freshwater goby larvae during short-term starvation. Bull. Jap. Soc. scient. Fish. 51: 707–709
Watanabe, Y., Sawada, N. (1985). Larval development of digestive organs and intestinal absorptive functions in the freshwater gobyChaenogobius annularis. Bull. Tohoku reg. Fish. Res. Lab. 47: 1–10
Zilversmit, D. B. (1978). Assembly of chylomicrons in the intestinal cell. In: Dietachy, J. M., Gotto, A. M., Ontko, J. A. (eds.) Disturbances in lipid and lipoprotein metabolism. American Physiological Society, Bethesda, Maryland, p. 69–81
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Communicated by J.M. Pérès, Marseille
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Deplano, M., Diaz, J.P., Connes, R. et al. Appearance of lipid-absorption capacities in larvae of the sea bassDicentrarchus labrax during transition to the exotrophic phase. Mar. Biol. 108, 361–371 (1991). https://doi.org/10.1007/BF01313645
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DOI: https://doi.org/10.1007/BF01313645