Abstract
Diel photosynthetic production of water-extractable and residual carbohydrates in natural phytoplankton populations was investigated by analyzing monosaccharide components with combined gas chromatography/mass spectrometry (GC/MS) and13C tracer methods. The applicability of this technique for measuring the photosynthetic production of natural phytoplankton populations was examined. Phytoplankton was collected in August 1984 from Kinu-ura Bay, Japan, and incubated under natural light and dark conditions for 24 h. Cell density and concentration of chlorophyll a increased about four- and three-fold, respectively, during the experimental period. Production of glucose, galactose, mannose, rhamnose, ribose, fucose, xylose and arabinose was measured in the water-extractable and residual carbohydrate fractions of the phytoplankton. Most of the monosaccharides in both fractions were produced the monosaccharides in both fractions were produced mainly during the daytime. Glucose was a major component in the water-extractable carbohydrate fraction produced by the phytoplankton, and decreased markedly at night. Thus, most of the glucose in the water-extractable carbohydrates seemed to be a constituent of the storage glucan of algal cells. Most monosaccharide components of the water-extractable carbohydrate fraction except glucose did not decrease significantly at night. Ribose of the water-extractable carbohydrate fraction was synthesized at a maximum rate in the morning, prior to the other monosaccharide constituents of this fraction. The major monosaccharide constituent in the residual carbohydrate fraction produced by the phytoplankton was glucose, which decreased significantly at night. Diel change in the production of monosaccharide constituents in residual carbohydrates indicated wide variability in the monosaccharide composition of cell-wall material in algae. The ratios of production of water-extractable and residual carbohydrates to POC production ranged from 8.8 to 28% and from 3.7 to 5.9%, respectively, throughout the daytime and nighttime.
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Literature cited
Allan, G. G., Lewin, J., Johnson, P. G. (1972). Marine polymers. IV. Diatom polysaccharides. Botanica. mar. 15: 102–108
Barlow, R. G. (1982). Phytoplankton ecology in the Southern Benguela current. III. Dynamics of a bloom. J. exp. mar. Biol. Ecol. 63: 239–248
Coombs, J., Volcani, B. E. (1968). Studies on the biochemistry and fine structure of silica-shell formation in diatoms. Chemical changes in the wall of Navicula pelliculosa during its formation. Planta 82: 280–292
Craig, H. (1953). The geochemistry of the stable carbon isotopes. Geochim. cosmochim. Acta 3: 53–92
Craigie, J. S. (1974). Storage products. In: Stewart, W. D. P. (ed.) Algal physiology and biochemistry. Blackwell Scientific Publications, Oxford, p. 206–235
Degens, E. T. (1976). Molecular mechanisms on carbonate, phosphate, and silica deposition in the living cell. Topics curr. Chem. 64: 1–112
Ford, C. W., Percival, E. (1965). The carbohydrates of Phaeodactylum tricornutum. Part I. Preliminary examination of the organism, and characterisation of low molecular weight material and of a glucan. J. chem. Soc. 5: 7035–7041
Hama, T., Handa, N. (1987). Pattern of organic matter production by natural phytoplankton population in a eutrophic lake. 1. Intracellular products. Arch. Hydrobiol. 109: 107–120
Hama, T., Handa, N., Hama, J. (1987). Determination of amino acid production rate of a marine phytoplankton population with13C and gas chromatography-mass spectrometry. Limnol. Oceanogr. 32: 1144–1153
Hama, T., Miyazaki, T., Ogawa, Y., Iwakuma, T., Takahashi, M., Otsuki, A., Ichimura, S. (1983). Measurement of photosynthetic production of a marine phytoplankton population using a stable13C isotope. Mar. Biol. 73: 31–36
Handa, N. (1969). Carbohydrate metabolism in the marine diatom Skeletonema costatum. Mar. Biol. 4: 208–214
Handa, N., Tominaga, H. (1969). A detailed analysis of carbohydrates in marine particulate matter. Mar. Biol 2: 228–235
Handa, N., Yanagi, K. (1969). Studies on water-extractable carbohydrates of the particulate matter from the northwest Pacific Ocean. Mar. Biol 4: 197–207
Heeky, R. E., Mopper, K., Kilham, P., Degens, E. T. (1973). The amino acid and sugar composition of diatom cell-walls. Mar. Biol. 19: 323–331
Kouchi, H. (1982). Direct analysis of13C abundance in plant carbohydrates by gas chromatography-mass spectrometry. J. Chromat. 241: 305–323
Mackie, W., Preston, R. D. (1974). Cell wall and intercellular region polysaccharides. In: Stewart, W. D. P. (ed.) Algal physiology and biochemistry. Blackwell Scientific Publications, Oxford, p. 40–85
Morris, I., Skea, W. (1978). Products of photosynthesis in natural populations of marine phytoplankton from the Gulf of Maine. Mar. Biol. 47: 303–312
Morris, I., Smith, A. E., Glover, H. E. (1981). Products of photosynthesis in phytoplankton off the Orinoco River and in the Caribbean Sea. Limnol. Óceanogr. 26: 1034–1044
Sakamoto, M., and co-authors (1984). Joint field experiments for comparisons of measuring methods of photosynthetic production. J. Plankton Res. 6: 365–383
SCOR (Scientific Committee on Oceanic Research working group 17) (1966). Determination of photosynthetic pigments in sea water. UNESCO monograph on oceanographic methodology, No. 1. UNESCO, Paris, p. 9–18
Slawyk, G., Minas, M., Collos, Y., Legendre, L., Roy, S. (1984). Comparison of radioactive and stable isotope tracer techniques for measuring photosyntheis.13C and14C uptake by marine phytoplankton. J. Plankton Res. 6: 249–257
Takeda, H., Hirokawa, T. (1978). Studies on the cell wall of Chlorella. I. Quantitative changes in cell wall polysaccharides during the cell cycle of Chlorella ellipsoidea. Pl. Cell Physiol., Tokyo 19: 591–598
Weiler, C. S., Karl, D. M. (1979). Diel changes in phased-dividing cultures of Ceratium furca (Dinophyceae): nucleotide triphosphates, adenylate energy charge, cell carbon, and patterns of vertical migration. J. Phycol. 15: 384–391
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Communicated by M. Anraku, Suva
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Hama, J., Handa, N. Diel photosynthetic production of cellular organic matter in natural phytoplankton populations, measured with13C and gas chromatography/mass spectrometry. Mar. Biol. 112, 175–181 (1992). https://doi.org/10.1007/BF00702459
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DOI: https://doi.org/10.1007/BF00702459