Abstract
Newly molted (0-d-old) cyprids of the barnacleBalanus amphitrite Darwin were prevented from settling for 0 to 14 d at four different temperatures (25, 20, 15 and 5°C treatments). The effect on settlement success of prolonging the cyprid lifetime was evaluated using a nitrilocellulose membrane assay. In addition, protein extract prepared from these cyprids was analyzed using gel electrophoresis to characterize the effect of age on protein content and composition. Settlement success was significantly affected for larvae aged at 25 (P < 0.001), 20 (P < 0.001) and 15°C (P < 0.05), while differences in settlement success between age groups was negligible at 5°C (P = 0.09). Settlement success of cyprids increased with time for up to 3 d (P < 0.001, Phase 1), following which settlement success significantly declined (P < 0.001, Phase 11). Temperature had no significant effect on settlement in Phase I (P = 0.17), but did enhance the decline in settlement success with age during Phase II (P < 0.001). Gel electrophoresis revealed a significant decline in the quantity of the cyprid storage protein CMP (Cyprid Major Protein) with increasing age at 25, 20 and 15°C, but CMP levels remained constant at 5°C. These results suggest that, upon molting to the cyprid stage, larvae may still require a settlement-competence attainment period. This may be achieved by CMP utilization during Phase I, depletion of which during Phase II may be responsible for reduction in settlement success with cyprid age such that remaining CMP stores can no longer support the production of adult structures following settlement.
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Clare AS, Freet RK, McClary M (1994) On the antennular secretion of the cyprid ofBalanus amphitrite amphitrite, and its role as settlement pheromone. J mar biol Ass UK 74: 243–250
Crisp DJ (1988) Reduced discrimination of laboratory-reared cyprids of the barnacleBalanus amphitrite amphitrite Darwin, Crustacea Cirripedea, with a description of a common abnormality. In: Thompson MF, Sarojini R, Nagabushanam R (eds) Marine biodeterioration. Oxford and IBH Publ. Co., New Delhi, pp 409–432
Crisp DJ, Meadows PS (1963) Adsorbed layers: the stimulus to settlement in barnacles. Proc R Soc (Ser B) 158: 364–387
Dineen JF, Hines AH (1992) Interactive effect of salinity and adult extract upon settlement of the estuarine barnacleBalanus improvisus (Darwin, 1854). J exp mar Biol Ecol 156: 239–252
Dineen JF, Hines AH (1994) Effects of salinity and adult extract on settlement of the oligohaline barnacleBalanus subalbidus. Mar Biol 119: 423–430
Hoeg JT, Ritchie LE (1987) Correlation between cyprid age, settlement rate and anatomical development inLernaeodiscus porcellanae (Cirripedea: Rhizocephala). J mar biol Ass UK 67: 65–75
Holland DL, Walker G (1975) The biochemical composition of the cypris larva of the barnacleBalanus balanoides L. J Cons int Explor Mer 36: 162–165
Kanost MR, Kawooya JK, Law JH, Ryan RO, Van Heusden MC, Ziegler R (1990) Insect haemolymph proteins. Adv Insect Physiol 22: 299–396
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, Lond 227: 680–685
Lucas MI, Walker G, Holland DL, Crisp DJ (1979) An energy budget for the free-swimming and metamorphosing larvae ofBalanus balanoides (Crustacea: Cirripedea). Mar Biol 55: 221–229
Maki JS, Rittschof D, Mitchell R (1992) Inhibition of larval barnacle attachment to bacterial films: an investigation of physical properties. Microb Ecol 23: 97–106
Matsumura K, Mori S, Nagano M, Fusetani N (1994) A protein complex, inducing larval settlement, from the barnacleBalanus amphitrite. Zool Sci 11 (Suppl): p 42
O'Connor NJ, Richardson DL (1994) Comparative attachment of barnacle cyprids (Balanus amphitrite Darwin, 1854;B. improvisus Darwin, 1854; &B. eberneus Gould, 1841) to polystyrene and glass substrata. J exp mar Biol Ecol 183: 213–225
Pechenik JA, Rittschof D, Schmidt AR (1993) Influence of delayed metamorphosis on survival and growth of juvenile barnaclesBalanus amphitrite. Mar Biol 115: 287–294
Rittschof D, Branscomb ES, Costlow JD (1984) Settlement and behavior in relation to flow and surface in larval barnacles,Balanus amphitrite, Darwin. J exp mar Biol Ecol 82: 131–146
Rittschof D, Hooper IR, Branscomb ES, Costlow JD (1985) Inhibition of barnacle settlement and behavior by natural products from whip corals,Leptogorgia virgulata (Lamarck, 1815). J chem Ecol 11: 551–563
Shimizu K, Satuito CG, Saikawa W, Fusetani N (1996) Larval storage protein of the barnacle,Balanus amphitrite: biochemical immunological similarities to vitellin. J exp Zool (in press)
Walley LJ (1969) Studies on the larval structure and metamorphosis ofBalanus balanoides (L.). Phil Trans R Soc (Ser B) 256: 237–280
Yule AB, Walker G (1985) Settlement ofBalanus balanoides: the effect of cyprid antennular secretion. J mar biol Ass UK 65: 707–712
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Communicated by T. Ikeda, Hakodate
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Satuito, C.G., Shimizu, K., Natoyama, K. et al. Age-related settlement success by cyprids of the barnacleBalanus amphitrite, with special reference to consumption of cyprid storage protein. Mar. Biol. 127, 125–130 (1996). https://doi.org/10.1007/BF00993652
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DOI: https://doi.org/10.1007/BF00993652