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Effects of triflusal and its main metabolite HTB on platelet interaction with subendothelium in healthy volunteers

  • Pharmacodynamics
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Abstract

The ex vivo effect of triflusal and acetylsalicylic acid (ASA) on platelet interaction with the subendothelium using the Baumgartner perfusion system (wall shear rate 350 s−1) was assessed in blood from 10 healthy volunteers who given a 15-day course of triflusal 600 mg per day and ASA 400 mg per day in a crossover trial.

The percentage of platelets on the subendothelium showed a decrease of 62% in samples from subjects on ASA and a decrease of 93% in those from subjects on triflusal (P<0.005). The percentage of the subendothelial surface covered by platelets was reduced by 23.3% after treatment with ASA, mainly due to inhibition of aggregates (75.2%), and by 29.9% after treatment with triflusal, mainly due to inhibition of aggregates (89.6%) and of adhesion (25%). The subendothelial surface covered by activated platelets (adhesions and thrombi) showed 32.5% inhibition after treatment with triflusal and 11.6% after treatment with ASA (P<0.043 vs. triflusal). In the in vitro experiments, 10 μmol·l−1 triflusal did not modify the percentage of the subendothelium covered by platelets. HTB 1 mmol·l−1 inhibited adhesion (26%) and aggregates (18%).

We conclude that HTB participates in the ex vivo effects of triflusal on the platelet-subendothelium interaction.

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Authors and Affiliations

  1. Department of Pharmacology, School of Medicine, Campus de Teatinos, E-29071, Málaga, Spain

    J. P. De La Cruz

  2. Department of Therapeutics, School of Medicine, University of Málaga, Málaga, Spain

    P. J. García & F. Sánchez de la Cuesta

  3. Department of Anatomy, School of Medicine, University of Málaga, Málaga, Spain

    M. A. Villalobos & J. M. Smith-Agreda

Authors
  1. J. P. De La Cruz
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  2. P. J. García
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  3. F. Sánchez de la Cuesta
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  4. M. A. Villalobos
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  5. J. M. Smith-Agreda
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De La Cruz, J.P., García, P.J., Sánchez de la Cuesta, F. et al. Effects of triflusal and its main metabolite HTB on platelet interaction with subendothelium in healthy volunteers. Eur J Clin Pharmacol 47, 497–502 (1995). https://doi.org/10.1007/BF00193701

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  • DOI: https://doi.org/10.1007/BF00193701

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