Skip to main content
SpringerLink
Log in

HSP72 induction of systemic organs of rats after severe burn injury

  • Originals
  • Published:
European Journal of Plastic Surgery Aims and scope Submit manuscript

Abstract

In order to understand the stress response of systemic organs to severe burn injury, the induction of 72-kD heat shock protein (HSP72) in various organs (brain, hypophysis, lung, heart, liver, pancreas, spleen, kidney, adrenal gland, and skeletal muscle) was investigated in rats with severe burns. A full-thickness burn was induced on the rats' skin by immersing the rats in hot water (90° C) for 3 s. At 0, 24, and 48 h after the burn injury, the HSP72 expression of various organs was examined using the Western blot analysis. At 24 h after the burn injury, the level of HSP72 had increased in the hypophysis, lung, heart, and kidney. In all organs examined, the expression of HSP72 had increased at 48 h after the burn injury. The level of HSP72 was highest in the hypophysis (3.3-fold compared to the control), and lowest in the brain and adrenal gland (1.7-fold of the control) at 48 h after the burn injury. These results confirm that severe burn injury causes a stress response in systemic organs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aikawa N, Martyn JA, Burke JF (1982) Pulmonary artery catheterization and thermodilution cardiac output determination in the management of critically burned patients. Am J Surg 135:811

    Google Scholar 

  2. Achauer BM, Allyn P, Furnas DW, Bartlett RH (1973) Pulmonary complications in burns: the major threat to the burn patient. Ann Surg 177:311

    Google Scholar 

  3. Baxter CR (1966) Serum myocardial depressant factor of burn shock. Surg Forum 17:1

    Google Scholar 

  4. Baxter CR, Shires T (1968) Physiological response to crystalloid resuscitation of severe burns. Ann NY Acad Sci 150:874

    Google Scholar 

  5. Blake MJ, Gershon D, Fargnoli J, Holbrook NJ (1990) Discordant expression of heat shock protein mRNA as in tissues of heat-stressed rats. J Biol Chem 265:15275

    Google Scholar 

  6. Blake MJ, Udelsman R, Feulner GJ, Norton DD, Holbrook NJ (1991) Stress-induced HSP70 expression in adrenal cortex: A glucocorticoid-sensitive, age-dependent response. Proc Natl Acad Sci USA 88:9873

    Google Scholar 

  7. Caldwell FT (1976) Energy metabolism following thermal burns. Arch Surg 111:181

    Google Scholar 

  8. Clark AS, Kelly RA, Mitch WE (1984) Systemic response to thermal injury in rats; Accelerated protein degradation and altered glucose utilization in muscle. J Clin Invest 74:888

    Google Scholar 

  9. Cosgrove JW, Brown IR (1983) Heat shock protein in mammalian brain and other organs after a physiologically relevant increase in body temperature induced by d-lysergic acid diethylamide. Proc Natl Acad Sci USA 80:569

    Google Scholar 

  10. Cuevas B, Kaufman AE, Fine JA (1973) Vasoactive agent in burns which produces fatal endotoxic shock. Surg Forum 24:39

    Google Scholar 

  11. Currie RW, White FP (1981) Trauma-induced protein in rat tissues: a physiological role for a “heat shock” protein? Science 214:72–73

    Google Scholar 

  12. Durkot MJ, Wolf RR (1981) Effects of adrenergic blockade on glucose kinetics in septic and burned guinea pigs. Am J Physiol 241:R222

    Google Scholar 

  13. Emami A, Schwarts JH, Borkan SC (1991) Transient ischemia or heat stress induces a cytoprotectant protein in rat kidney. Am J Physiol 260:F479

    Google Scholar 

  14. Gilmore JP, Handford SW (1956) Hemodynamic response of the dog to thermal radiation. J Appl Physiol 8:393

    Google Scholar 

  15. Knowlton AA, Brecher P, Apstein CS (1991) Rapid expression of heat shock protein in the rabbit after brief cardiac ischemia. J Clin Invest 87:139

    Google Scholar 

  16. Lindquist S (1986) The heat-shock response. Annu Rev Biochem 55:1151

    Google Scholar 

  17. Mivech NH, Monson JM, Hahn GM (1991) Expression of HSP-28 and three HSP-70 genes during the development and decay of thermotolerance in leukemic and nonleukemic human tumors. Cancer Res 51:6608

    Google Scholar 

  18. Moati F, Sepulchre C, Miskulin M, Huisman O, Moczar E, Robert A, Monteil R, Guilbaud J (1979) Biochemical and pharmacological properties of a cardiotoxic factor isolated from the blood serum of burned patients. J Pathol 127:147

    Google Scholar 

  19. Muramatsu T, Tada H, Kobayashi N, Yamaji M, Shirai T, Ohnishi T (1992) Induction of the 72-kD heat shock protein in organ-cultured normal human skin. J Invest Dermatol 98:786

    Google Scholar 

  20. Ogawa Y, Matsumoto K, Ofuji S (1977) Changes in adenine nucleotide and mitochondrial metabolism of the kidney of burned rats and their relation to insulin. J Lab Clin Med 90:457

    Google Scholar 

  21. Schlesinger MJ (1990) Heat shock proteins. J Biol Chem 265:12111

    Google Scholar 

  22. Seiye H (1946) The general adaptation syndrome and the diseases of adaptation. J Clin Endocrinol Metab 6:117

    Google Scholar 

  23. Sepulchre C, Moati F, Miskulin M, Husman O, Moczar E, Robert A, Monteil R, Guilbaud J (1979) Biochemical and pharmacological properties of a neurotoxic protein isolated from the blood serum of heavily burned patients. J Pathol 127:137

    Google Scholar 

  24. Udersman R, Blake MJ, Holbrook NJ (1991) Molecular response to surgical stress: Specific and simultaneous heat shock protein induction in the adrenal cortex, aorta, and vena cava. Surgery 110:1125

    Google Scholar 

  25. Udelsman R, Blake MJ, Stagg CA, Li D, Putney J, Holbrook NJ (1993) Vascular heat shock protein expression in respone to stress-endocrine and automatic regulation of this age-dependent response. J Clin Invest 91:465

    Google Scholar 

  26. Udelsman R, Goldstein Ds, Loriaux DL, Chrousos GP (1987) Catecholamine — glucocorticoid interactions during surgical stress. J Surg Res 43:539

    Google Scholar 

  27. Vass K, Welch WJ, Nowak TS (1988) Localization of 70-kDa stress protein induction in gerbil brain after ischemia. Acta Neuropathol 77:128

    Google Scholar 

  28. Vaughan GM, Becker RA, Allen JP, Goodwin CW, Pruitt BA, Mason AD (1982) Cortisol and corticotropin in burned patients. J Trauma 22:263

    Google Scholar 

  29. Volenec FJ, Clark GM, Mani MM, kyner J, Humphrey LJ (1979) Metabolic profiles of thermal trauma. Ann Surg 190:694

    Google Scholar 

  30. Welch WJ, Suhan JP (1986) Cellular and biochemical events in mammalian cells during and recovery from physiological stress. J Cell Biol 103:2035

    Google Scholar 

  31. Wilmore DW, Aulick LH, Mason AD, Pruitt BA (1977) Influence of the burn wound on local and systemic responses to injury. Ann Surg 186:444

    Google Scholar 

  32. Wilmore DW, Long JM, Mason AD, Skreen RW, Pruitt BA (1974) Mediator of hypermetabolic response to thermal injury. Ann Surg 180:653

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Plastic Surgery, Nara Medical University, 634, Kashihara, Nara, Japan

    M. Hatoko, S. Hirai & H. Tada

  2. Department of Dermatology, Nara Medical University, 634, Kashihara, Nara, Japan

    T. Muramatsu & T. Shirai

  3. Department of Biology, Nara Medical University, 634, Kashihara, Nara, Japan

    H. Matsumoto & T. Ohnishi

Authors
  1. M. Hatoko
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Hirai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Tada
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Muramatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Shirai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T. Ohnishi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hatoko, M., Hirai, S., Tada, H. et al. HSP72 induction of systemic organs of rats after severe burn injury. Eur J Plast Surg 20, 136–140 (1997). https://doi.org/10.1007/BF01002047

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01002047

Key words

Search

Navigation