Summary
Gossypol acetic acid in a concentration of 1,000 μg/ml solvent is able to immobilize 1 ml of native bull semen (sperm concentration: 8.5×108/ml; motility rate: 87.4%) within 30 min. After GAA treatment the spermatozoa show severe morphological damage on the membrane system, on the acrosomal complex and on the tubular complex of the end piece. The working mechanism of GAA can be assumed to be inactivation of enzyme activities or in direct reactions with plasma membrane material.
Similar content being viewed by others
References
Bozek A, Jensen DR, Tone JN (1981) Scanning electron microscopic study from gossypol-treated rats. Cell Tissue Res 219:659–663
Chang MC, Gu Z, Saksena SK (1980) Effect of gossypol on fertility of male rats, hamsters and rabbits. Contraception 21: 461–469
Hadley MA, Lin CY, Dym M (1981) Effects of gossypol on the reproductive system of male rats. J Androl 2:190–199
Hahn DW, Rusticus C, Probst A, Hamm R (1981) Antifertility and endocrine activities of gossypol on rodents. Contraception 24:97–105
Kalla NR, Vasudev M (1981) Studies on the male antifertility agent, gossypol acetic acid: II. Effect of gossypol acetic acid on the motility and ATP-ase activity of human spermatozoa. Andrologia 13:95–98
Kalla NR, Vasudev M, Arora G (1981) Studies on the male antifertility agent — gossypol acetic acid. III. Effect of gossypol acetic acid on rat testes. Andrologia 13:242–249
Lee CY, Malling HY (1981) Selective inhibition of sperm specific lactate dehydrogenase X by an antifertility agent, gossypol. Fed Proc 40:718–723
Nadakavukaren MJ, Sorensen RH, Tore JN (1979) Effect of gossypol on the ultrastructure of rat spermatozoa. Cell Tissue Res 204:293–296
National Coordinating Group of Male Antifertility Agents (1978) Gossypol — A new antifertility agent for males. Chin Med J (English edition) 4:417–428
Pösö J, Wichmann K, Jänne J, Luukkainen T (1980) Gossypol, a powerful inhibitor of human spermatozoa metabolism. Lancet I:885–886
Rovan E, Simonsberger P (1974) Die Agarröhrchenmethode für elektronenmikroskopische Präparation von Zellsuspensionen und kleinen Gewebestückchen. Mikroskopie 30:129–134
Tso WW, Lee CS (1981) Effect of gossypol on boar spermatozoa in vitro. Arch Androl 7:85–88
Waller DP, Zaneveld LJ, Fong HHS (1980) In vitro spermacidal activity of gossypol. Contraception 22:(2)183–187
Weinbauer GF, Rovan E, Frick J (1982) Antifertility efficacy of gossypol acetic acid in male rats. Andrologia 14:270–275
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rovan, E., Kalla, N.R., Frick, J. et al. Effect of gossypol on bull spermatozoa in vitro. Urol. Res. 12, 187–192 (1984). https://doi.org/10.1007/BF00255921
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00255921