Summary
In order to have a rapid method of measuring the inhibition of calcium-oxalate monohydrate growth in freshly voided whole urine, a test system by Meyer and Smith that has originally been developed for diluted urine was modified. The crystallization processes were monitored by an ion-selective calcium electrode, which allowed determination of the half-life value of the decrease in calcium within 25 min. Even given the high inhibitory activity of whole urine, the test gave reliable results when a high seed concentration was used. Inhibition was expressed as the ratio between the half-lives of the calcium decrease obtained in the presence and in the absence of inhibitors. This approach allowed kinetic studies of individual inhibitors in model solutions. Furthermore, the measurements of inhibitors in urine could be, performed before the chemical composition was determined.
Similar content being viewed by others
References
Ackermann D, Brown CM, Purich DL (1989) Measurements of urinary supersaturation, creation, of standard supersaturations. Urol Res 17:144
Baumann JM (1979) Experience with the measurement of inhibitory activity of urine and crystallization inhibitors by different, techniques. Urol Res 7:183
Baumann JM (1988) How to measure crystallization conditions in urine: a comparison of 7 methods. Urol Res 16:137
Baumann JM, Ackermann D, Affolter B (1989) The influence of hydroxyapatite and pyrophosphate on the formation product of calcium oxalate at different pHs. Urol Res 17:153
Meyer JL, Smith LH (1975) Growth of calcium oxalate crystals I and II. Invest Urol 13:31
Nielsen AE, Christoffersen J (1982) The mechanisms of crystal growth and dissolution. In: Nancollas GH (ed) Biological mineralization and demineralization. Springer, Berlin Heidelberg, New York, p 37
Robertson WG, Hughes H, Barkworth SA, Walker VR (1988) Competition between the known inhibitors and promoters of calcium oxalate crystallization in urine. In: Martelli A, Buli P, Marchesini B (eds) Inhibitors of crystallization in renal lithiasis and their clinical application. Acta Medica, Rome, p 45
Ryall RL, Grover PK, Doyle IR, Harnett RM, Hibbered CM, Edyvane KA, Marshall VR (1988) The effect of macromolecules on the crystallization of calcium oxalate in human urine. In: Martelli A, Buli P, Marchesini B (eds) Inhibitors of crystallization in renal lithiasis and their clinical application. Acta Medica, Rome, p 51
sheehan ME, Nancollas GH (1980) Calcium oxalate crystal growth. A new constant composition method for modelling urinary stone formation. Invest Urol 17:446
Springman KE, Drach GW, Gottung B, Randolph AD (1986) Effects of human urine on aggregation of calcium oxalate crystals. J Urol 135:69
Werness PG, Brown CM, Smith LH, Finalyson B (1985) EQUIL II: a BASIC computer program for the calculation of urinary saturation. J Urol 134:1242
Will EJ, Bijvoet OLM, Blomen LJMJ, Van der Linden H (1983) Growth kinetics of calcium oxalate monohydrate I-III. J Crystal Growth 64:297
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Baumann, J.M., Ackermann, D. & Affolter, B. Rapid method of measuring the inhibition of calcium-oxalate monohydrate growth in urine. Urol. Res. 18, 219–222 (1990). https://doi.org/10.1007/BF00295851
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00295851